1887

Abstract

A novel species is described on the basis of phenotypic, chemotaxonomic and phylogenetic studies. Four halophilic organisms were isolated from marine sand and marine macroalgae samples by using high-pH marine agar 2216. An analysis of the nearly complete 16S rRNA gene sequences of these new isolates indicated that they were phylogenetically close (16S rRNA gene sequence similarity >99·5 %, gene sequence similarity >97·8 %), and were most closely related to (16S rRNA gene sequence similarity 97·1–97·3 %, gene sequence similarity 84·4–85·0 %). Chemotaxonomic data (major menaquinone MK7; major fatty acids C and C 9) supported the affiliation of the new isolates to the genus . The results of physiological and biochemical tests allowed phenotypic differentiation of the isolates from . It is therefore proposed that the new isolates represent a novel species with the name sp. nov. and type strain A4D-4 (=MBIC06480=DSM 16917).

Keyword(s): NJ, neighbour-joining
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63689-0
2005-09-01
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/5/ijs551851.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63689-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Andersson, B. A. & Holman, R. T. ( 1974; ). Pyrrolidides for mass spectrometric determination of the position of the double bond in monounsaturated fatty acids. Lipids 9, 185–190.[CrossRef]
    [Google Scholar]
  3. Cole, J. R., Chai, B., Marsh, T. L. & 8 other authors ( 2003; ). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Haller, C. M., Rölleke, S., Vybiral, D., Witte, A. & Velimirov, B. ( 2000; ). Investigation of 0·2-micron-filterable bacteria from the Western Mediterranean Sea using a molecular approach: dominance of potential starvation forms. FEMS Microbiol Ecol 31, 153–161.
    [Google Scholar]
  6. Kostka, J. E. & Nealson, K. H. ( 1998; ). Isolation, cultivation, and characterization of iron- and manganese-reducing bacteria. In Techniques in Microbial Ecology, pp. 58–78. Edited by R. S. Burlage, R. Atlas, D. Stahl, G. Geesey & G. Sayler. New York: Oxford University Press.
  7. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: Molecular Evolutionary Genetics Analysis Software. Tempe, AZ: Arizona State University.
  8. Lovley, D. R. & Phillips, E. J. ( 1986; ). Organic matter mineralization with reduction of ferric iron in anaerobic sediments. Appl Environ Microbiol 51, 683–689.
    [Google Scholar]
  9. Murray, R. G. E., Doetsch, R. N. & Robinow, C. F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  10. Nishijima, M., Araki-Sakai, M. & Sano, H. ( 1997; ). Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28, 113–122.[CrossRef]
    [Google Scholar]
  11. Rosselló-Mora, R. A., Ludwig, W., Kämpfer, P., Amann, R. & Schleifer, K.-H. ( 1995; ). Ferrimonas balearica gen. nov., spec. nov., a new marine facultative Fe(III)-reducing bacterium. Syst Appl Microbiol 18, 196–202.[CrossRef]
    [Google Scholar]
  12. Sakane, T. & Kuroshima, K. ( 1997; ). Viabilities of dried cultures of various bacteria after preservation for 20 years and their production by the accelerated storage test. Microbiol Cult Collect 13, 1–7.
    [Google Scholar]
  13. Satomi, M., Oikawa, H. & Yano, Y. ( 2003; ). Shewanella mariniintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella saire sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. Int J Syst Evol Microbiol 53, 491–499.[CrossRef]
    [Google Scholar]
  14. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  15. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  18. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  19. Yamamoto, S. & Harayama, S. ( 1995; ). PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61, 1104–1109.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63689-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63689-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error