1887

Abstract

A Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, Slu-01, was isolated from activated sludge from a wastewater treatment plant in Daejeon, South Korea. After 3 days on R2A medium, strain Slu-01 developed colourless colonies (0·7–1·2 mm). The non-motile rods (0·7–1·0×1·0–2·0 μm) were slightly curved and occurred singly and in pairs. No filamentous cells were found. On the basis of 16S rRNA gene sequence similarity, strain Slu-01 was shown to belong to the family and was most closely related to (96·6 %) and (96·5 %). The genomic DNA G+C content of strain Slu-01 was 68·1 mol%, within the range of 67·3–68·4 mol% for the genus . Chemotaxonomic data (major ubiquinone, Q-10; major fatty acids C 7, C, C and C) and 16S rRNA gene sequence analysis supported the affiliation of strain Slu-01 to the genus . However, DNA–DNA hybridization data and phenotypic properties showed that strain Slu-01 could be distinguished from the two other species with validly published names. Thus, it is suggested that strain Slu-01 represents a novel species of the genus , for which the name is proposed. The type strain is Slu-01 (=KCTC 12206=IAM 15119).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63687-0
2005-09-01
2020-01-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/5/ijs552001.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63687-0&mimeType=html&fmt=ahah

References

  1. Abraham, W. R., Strompl, C., Meyer, H. & 8 other authors ( 1999; ). Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. Int J Syst Bacteriol 49, 1053–1073.[CrossRef]
    [Google Scholar]
  2. Atlas, R. M. ( 1993; ). Handbook of Microbiological Media. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  3. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  4. Cappuccino, J. G. & Sherman, N. ( 2002; ). Microbiology: a Laboratory Manual, 6th edn. Menlo Park, CA, USA: Benjamin Cummings.
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  8. Im, W.-T., Bae, H.-S., Yokota, A. & Lee, S.-T. ( 2004; ). Herbaspirillum chlorophenolicum sp. nov., a 4-chlorophenol-degrading bacterium. Int J Syst Evol Microbiol 54, 851–855.[CrossRef]
    [Google Scholar]
  9. Kanso, S. & Patel, B. K. C. ( 2004; ). Phenylobacterium lituiforme sp. nov., a moderately thermophilic bacterium from a subsurface aquifer, and emended description of the genus Phenylobacterium. Int J Syst Evol Microbiol 54, 2141–2146.[CrossRef]
    [Google Scholar]
  10. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  11. Komagata, K. & Suzuki, K. I. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  12. Kumar, S., Tamura, K., Jacobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  13. Lewin, R. A. & Lounsbery, D. M. ( 1969; ). Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58, 145–170.[CrossRef]
    [Google Scholar]
  14. Lingens, F., Blecher, R., Blecher, H., Blobel, F., Eberspächer, J., Fröhner, C., Görisch, H., Görisch, H. & Layh, G. ( 1985; ). Phenylobacterium immobile gen. nov., sp. nov., a Gram-negative bacterium that degrades the herbicide chloridazon. Int J Syst Bacteriol 35, 26–39.[CrossRef]
    [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Reva, O. N., Vyunitskaya, V. A., Reznik, S. R., Kozachko, I. A. & Smirnov, V. V. ( 1995; ). Antibiotic susceptibility as a taxonomic characteristic of the genus Bacillus. Int J Syst Bacteriol 45, 409–411.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sasser, M. ( 1990; ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101. Newark, DE: MIDI.
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Ten, L. N., Im, W.-T., Kim, M.-K., Kang, M.-S. & Lee, S.-T. ( 2004; ). Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 56, 375–382.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63687-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63687-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 2001 - 2005

. [PDF](50 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error