1887

Abstract

sp. strain SMA, isolated in Poland from white satin moth larvae, L. (Lepidoptera: Lymantriidae), was serologically distinct from other species, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a single cell membrane (lacking a cell wall). Growth of strain SMA occurred in M1D medium at 30 °C. Strain SMA catabolized both glucose and arginine, but did not hydrolyse urea. The G+C content of the DNA was 24±1 mol% as determined by melting temperature analysis. Serological analysis revealed a very weak cross-reactivity (positive reaction only up to a 1 : 80 dilution) with two strains, 277F ( sp. group I-4) and LB-12 ( sp. group I-5). Strain SMA (=ATCC BAA-521=NBRC 100392) is designated the type strain of a novel species, sp. nov. (class : order : family ).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63685-0
2005-11-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/6/2447.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63685-0&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittler R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for characterization of Mycoplasma species. Int J Syst Bacteriol 20:35–58 [CrossRef]
    [Google Scholar]
  2. Carle P., Saillard C., Bové J. M. 1983; Determination of guanine plus cytosine content of DNA. Methods Mycoplasmol 1:301–308
    [Google Scholar]
  3. Gasparich G. E., Hackett K. J., Clark E. A., Renaudin J., Whitcomb R. F. 1993; Occurrence of extrachromosomal deoxyribonucleic acids in spiroplasmas associated with plants, insects, and ticks. Plasmid 29:81–93 [CrossRef]
    [Google Scholar]
  4. Gasparich G. E., Whitcomb R. F., Dodge D., French F. E., Glass J., Williamson D. L. 2004; The genus Spiroplasma and its non-helical descendants: phylogenetic classification, correlation with phenotype and roots of the Mycoplasma mycoides clade. Int J Syst Evol Microbiol 54:893–918 [CrossRef]
    [Google Scholar]
  5. Hackett K. J., Clark T. B. 1989; The ecology of spiroplasmas. In The Mycoplasmas vol. 5 pp  113–200 Edited by Whitcomb R. F., Tully J. G. New York: Academic Press;
    [Google Scholar]
  6. Hackett K. J., Whitcomb R. F., Henegar R. B., Wagner A. C., Clark E. A., Tully J. G., Molina F., McKay W., Santini P. 1990; Mollicute diversity in arthropod hosts. Zentralbl Bakteriol Suppl 20:441–454
    [Google Scholar]
  7. Hackett K. J., Whitcomb R. F., Clark T. B. 12 other authors 1996; Spiroplasma leptinotarsae sp. nov., a mollicute uniquely adapted to its host, the Colorado potato beetle, Leptinotarsa decemlineata (Coloeoptera: Chrysomelidae. Int J Syst Bacteriol 46:906–911 [CrossRef]
    [Google Scholar]
  8. Humphreys N. 1996 Satin Moth in British Columbia . Forest pest leaflet 38 Victoria, BC: Natural Resources Canada; Canadian Forest Service, Pacific Forestry Centre:
    [Google Scholar]
  9. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes 1995; Revised minimum standards for description of new species of the class Mollicutes (division Tenericutes . Int J Syst Bacteriol 45:605–612 [CrossRef]
    [Google Scholar]
  10. Konai M., Clark E. A., Camp M., Koch A. L., Whitcomb R. F. 1996a; Temperature ranges, growth optima, and growth rates of Spiroplasma ( Spiroplasmataeae , class Mollicutes ) species. Curr Microbiol 32:314–319 [CrossRef]
    [Google Scholar]
  11. Konai M., Hackett K. J., Williamson D. L., Lipa J. J., Pollack J. D., Gasparich G. E., Clark E. A., Vacek D. C., Whitcomb R. F. 1996b; Improved cultivation systems for isolation of the Colorado Potato Beetle Spiroplasma. Appl Environ Microbiol 62:3453–3458
    [Google Scholar]
  12. Lipa J. J., Ziemnicka J., Gazecka B., Korytek L. 1988; A newly recorded spiroplasma infection in Stilpnotia salicis L. ( Lepidoptera, Lymantriidae . Pr Nauk Inst Ochr Rosl 30:15–18
    [Google Scholar]
  13. Maddison W. P., Maddison D. R. 1992 macclade: analysis of phylogeny and character evolution, version 3.0 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  14. Rose D. L., Tully J. G., Bové J. M., Whitcomb R. F. 1993; A test for measuring growth responses of mollicutes to serum and polyoxyethylene sorbitan. Int J Syst Bacteriol 43:527–532 [CrossRef]
    [Google Scholar]
  15. Smith P. F., Lecce J. G., Lynn R. J. 1954; A lipoprotein as a growth factor for certain pleuropneumonia-like organisms. J Bacteriol 68:627–633
    [Google Scholar]
  16. Swofford D. L. 1998 paup – Phylogenetic Analysis Using Parsimony and other methods, version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Tully J. G. 1983a; Cloning and filtration techniques for mycoplasmas. Methods Mycoplasmol 1:173–177
    [Google Scholar]
  19. Tully J. G. 1983b; Tests for digitonin sensitivity and sterol requirement. Methods Mycoplasmol 1:355–362
    [Google Scholar]
  20. Whitcomb R. F. 1983; Culture and media for spiroplasmas. Methods Mycoplasmol 1:147–158
    [Google Scholar]
  21. Williamson D. L. 1983; Specialized electron microscopic techniques for spiroplasmas in plant and insect tissues. Methods Mycoplasmol 1:71–76
    [Google Scholar]
  22. Williamson D. L., Whitcomb R. F. 1983; Special serological tests for spiroplasma identification. Methods Mycoplasmol 2:249–259
    [Google Scholar]
  23. Williamson D. L., Whitcomb R. F., Tully J. G. 1978; The spiroplasma deformation test, a new serological method. Curr Microbiol 1:203–207 [CrossRef]
    [Google Scholar]
  24. Williamson D. L., Whitcomb R. F., Tully J. G. 10 other authors 1998; Revised group classification of the genus Spiroplasma . Int J Syst Bacteriol 48:1–12 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63685-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63685-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error