A Gram-negative, rod-shaped, urea-dissolving and non-spore-forming bacterium, designated strain NiVa 51, was isolated from water of the River Torsa in Hasimara, Jalpaiguri district, West Bengal, India. On the basis of 16S rRNA gene sequence similarity, strain NiVa 51 was shown to belong to the - and to be related to subsp. (98·35 %) and subsp. (98·30 %); however, strain NiVa 51 exhibited only 43·7 % similarity to by DNA–DNA hybridization. The G+C content of the genomic DNA of the isolate was 60 mol%. Both biochemical characteristics and fatty acid analysis data supported the affiliation of strain NiVa 51 to the genus . Furthermore, strain NiVa 51 was found to utilize urea as nitrogen source. The results of DNA–DNA hybridization as well as physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain NiVa 51 from recognized species. Strain NiVa 51 therefore represents a novel species, for which the name sp. nov. is proposed, with type strain NiVa 51 (=LMG 22860=CCUG 50595).


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vol. , part 5, pp. 2155 – 2158

Growth curve of strain NiVa 51

Phylogenetic trees derived from 16S rRNA gene sequences

Cellular fatty acid composition of strain NiVa 51 and phylogenetically related taxa in the same genus

Similarity coefficient ( ) values of taxa of members of the family

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