1887

Abstract

Two bacterial strains, designated D-1,5a and D-1,5b, were isolated from a medieval wall painting in the chapel of Castle Herberstein, Styria (Austria). The Gram-positive, heterotrophic, aerobic, spore-forming rods showed nearly identical whole-cell protein patterns, identical genomic fingerprints and identical physiological profiles, demonstrating their relationship at the species level. Both strains contained -diaminopimelic acid in their peptidoglycan, possessed a quinone system comprising menaquinone MK-7 and had fatty acid profiles in which C iso and C anteiso were predominant. The 16S rRNA gene sequence of D-1,5a showed the highest similarity (99·5 %) to the sequence of sp. LMG 20243, and IFO 15715 was the next most closely related established species (96·5 %). Other type strains, such as DSM 91, SD/3, JG-30, IAM 13418, DSM 6308, LMG 21833 and LMG 21838, shared 96·0–96·1 % 16S rRNA gene sequence similarity with D-1,5a. The combination of physiological and chemotaxonomic traits distinguishes the two strains from those species sharing the highest sequence similarities (96·0–96·5 %). On the basis of these characteristics and the phylogenetic position of strain D-1,5a (=DSM 16534=CCM 7228), this strain is assigned as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63660-0
2005-09-01
2024-10-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/5/ijs552119.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63660-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  2. Auling G., Probst A., Kroppenstedt R. M. 1985; Chemo- and molecular taxonomy of d(−)-tartrate-utilizing pseudomonads. Syst Appl Microbiol 8:114–120
    [Google Scholar]
  3. Bishop D. G., Rutberg L., Samuelsson B. 1967; The chemical composition of the cytoplasmic membrane of Bacillus subtili s. Eur J Biochem 2:448–453 [CrossRef]
    [Google Scholar]
  4. Brundish D. E., Shaw N., Baddiley J. 1965; The occurrence of glycolipids in Gram-positive bacteria. Biochem J 95:21c–22c
    [Google Scholar]
  5. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872. In Bergey's Manual of Systematic Bacteriology vol 2 pp  1105–1140 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  7. Gorbushina A. A., Heyrman J., Dornieden T., Gonzalez-Delvalle M., Krumbein W. E., Laiz L., Petersen K., Saiz-Jiminez C., Swings J. 2004; Bacterial and fungal diversity and biodeterioration problems in mural painting environments of St. Martins church (Greene-Kreiensen, Germany). Int Biodeter Biodegradation 5313–24 [CrossRef]
  8. Gurtner C., Heyrman J., Piñar G., Lubitz W., Swings J., Rölleke S. 2000; Comparative analyses of the bacterial diversity on two different biodeteriorated wall paintings by DGGE and 16S rDNA sequence analysis. Int Biodeter Biodegradation 46:229–239 [CrossRef]
    [Google Scholar]
  9. Hall T. A. 1999; bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  10. Heyrman J., Balcaen A., De Vos P., Schumann P., Swings J. 2002a; Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria. Int J Syst Evol Microbiol 52:1641–1646 [CrossRef]
    [Google Scholar]
  11. Heyrman J., Balcaen A., De Vos P., Swings J. 2002b; Halomonas muralis sp. nov., isolated from microbial biofilms colonizing the walls and murals of the Saint-Catherine chapel (Castle Herberstein, Austria. Int J Syst Evol Microbiol 52:2049–2054 [CrossRef]
    [Google Scholar]
  12. Heyrman J., Balcaen A., Rodriguez-Diaz M., Logan N. A., Swings J., De Vos P. 2003a; Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria. Int J Syst Evol Microbiol 53:459–463 [CrossRef]
    [Google Scholar]
  13. Heyrman J., Logan N. A., Busse H.-J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P. 2003b; Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus , as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus . Int J Syst Evol Microbiol 53:501–511 [CrossRef]
    [Google Scholar]
  14. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodriguez-Diaz M., Felske A., De Vos P. 2004; Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov.and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54:47–57 [CrossRef]
    [Google Scholar]
  15. Kämpfer P. 1994; Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst Appl Microbiol 17:86–98 [CrossRef]
    [Google Scholar]
  16. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  17. Kaneko T., Katoh K., Fujimoto M., Kumagi M., Tamaoka J., Katayama-Fujimura Y. 1986; Determination of the nucleotide composition of a deoxyribonucleic acid by high-performance liquid chromatography of its enzymatic hydrolysates: a review. J Microbiol Methods 4:229–240 [CrossRef]
    [Google Scholar]
  18. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  19. Minnikin D. E., Abdolrahimzadeh H., Baddiley J. 1974; The occurrence of phosphatidylethanolamine and glycosyl diglycerides in thermophilic bacilli. J Gen Microbiol 83:415–418 [CrossRef]
    [Google Scholar]
  20. Nagel M., Andreesen J. R. 1991; Bacillus niacini sp. nov., a nicotinate-metabolizing mesophile isolated from soil. Int J Syst Bacteriol 41:134–139 [CrossRef]
    [Google Scholar]
  21. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [CrossRef]
    [Google Scholar]
  22. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  23. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus . J Gen Microbiol 134:1847–1882
    [Google Scholar]
  24. Schleifer K.-H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  25. Sneath P. H. A. 1986; Endospore-forming gram-positive rods and cocci. In Bergey's Manual of Systematic Bacteriology vol 2 pp  1104–1207 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  26. Suresh K., Prabagaran S. R., Sengupta S., Shivaji S. 2004; Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54:1369–1375 [CrossRef]
    [Google Scholar]
  27. Täubel M., Kämpfer P., Buczolits S., Lubitz W., Busse H.-J. 2003; Bacillus barbaricus sp. nov., isolated from an experimental wall painting. Int J Syst Evol Microbiol 53:725–730 [CrossRef]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  29. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  30. Ventosa A., Marquez M. C., Kocur M., Tindall B. J. 1993; Comparative study of “ Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . Int J Syst Bacteriol 43:245–248 [CrossRef]
    [Google Scholar]
  31. Wieser M., Busse H.-J. 2000; Rapid identification of Staphylococcus epidermidis . Int J Syst Evol Microbiol 50:1087–1093 [CrossRef]
    [Google Scholar]
  32. Wieser M., Schumann P., Martin K., Altenburger P., Burghardt J., Lubitz W., Busse H.-J. 1999; Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Bacteriol 49:1165–1170 [CrossRef]
    [Google Scholar]
  33. Wieser M., Denner E. M. B., Kämpfer P. 10 other authors 2002; Emended descriptions of the genus Micrococcus , Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al . 1974). Int J Syst Evol Microbiol 52:629–637
    [Google Scholar]
  34. Yoon J.-H., Lee C.-H., Oh T.-K. 2005; Bacillus cibi sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 55:733–736 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63660-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63660-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error