1887

Abstract

Two bacterial strains, designated D-1,5a and D-1,5b, were isolated from a medieval wall painting in the chapel of Castle Herberstein, Styria (Austria). The Gram-positive, heterotrophic, aerobic, spore-forming rods showed nearly identical whole-cell protein patterns, identical genomic fingerprints and identical physiological profiles, demonstrating their relationship at the species level. Both strains contained -diaminopimelic acid in their peptidoglycan, possessed a quinone system comprising menaquinone MK-7 and had fatty acid profiles in which C iso and C anteiso were predominant. The 16S rRNA gene sequence of D-1,5a showed the highest similarity (99·5 %) to the sequence of sp. LMG 20243, and IFO 15715 was the next most closely related established species (96·5 %). Other type strains, such as DSM 91, SD/3, JG-30, IAM 13418, DSM 6308, LMG 21833 and LMG 21838, shared 96·0–96·1 % 16S rRNA gene sequence similarity with D-1,5a. The combination of physiological and chemotaxonomic traits distinguishes the two strains from those species sharing the highest sequence similarities (96·0–96·5 %). On the basis of these characteristics and the phylogenetic position of strain D-1,5a (=DSM 16534=CCM 7228), this strain is assigned as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2005-09-01
2019-12-13
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Genomic fingerprints generated after ERIC-PCR for strains D-1,5a (middle) and D-1,5b (right). Markers are shown in the left lane.

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Polar lipid profile of strain D-1,5a after two-dimensional TLC and detection with molybdatophosphoric acid. Abbreviations: DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; GL1-2, unidentified glycolipids; L1-4, unidentified polar lipid; PL1-2, unidentified phospholipids; bPi, brownish pigment.

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