1887

Abstract

An alkaliphilic, slightly halotolerant, chemo-organotrophic, Gram-positive, rod-shaped bacterium, strain 69B4, was isolated from the sediment of the littoral zone of Lake Bogoria, Kenya. Phylogenetically, it is a member of the genus , showing less than 97·5 % sequence similarity to the type strains of other species. The highest level of similarity, albeit moderate, was found with respect to DSM 20118. Chemotaxonomic properties confirm the 16S rRNA gene-based generic affiliation, i.e. a DNA G+C content of 71·5 mol%, anteiso-C and C as the major fatty acids, MK-9(H) as the major isoprenoid quinone, a peptidoglycan containing -ornithine as the diamino acid and -aspartic acid in the interpeptide bridge and phosphatidylglycerol as the only identified main polar lipid. The strain is aerobic to facultatively anaerobic, being capable of growth under strictly anaerobic conditions. Optimal growth occurs between pH values 9·0 and 10·0. On the basis of its distinct phylogenetic position and metabolic properties, strain 69B4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 69B4 (=DSM 16987=CIP 108683).

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2005-07-01
2024-04-23
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References

  1. Bagnara C., Toci R., Gaudin C., Belaich J. P. 1985; Isolation and characterization of a cellulolytic microorganism, Cellulomonas fermentans sp. nov. Int J Syst Bacteriol 35:502–507 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Pascual C. 2000; Reclassification of Actinomyces humiferus (Gledhill and Casida) as Cellulomonas humilata nom. corrig., comb. nov. Int J Syst Evol Microbiol 50:661–663 [CrossRef]
    [Google Scholar]
  3. De Soete G. 1983; A least square algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626 [CrossRef]
    [Google Scholar]
  4. Duckworth A. W., Grant W. D., Jones B. E., van Steenbergen R. 1996; Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19:181–191 [CrossRef]
    [Google Scholar]
  5. Elberson M. A., Malekzadeh F., Yazdi M. T., Kameranpour N., Noori-Daloii M. R., Matte M. H., Shahamat M., Colwell R. R., Sowers K. R. 2000; Cellulomonas persica sp. nov. and Cellulomonas iranensis sp. nov., mesophilic cellulose-degrading bacteria isolated from forest soils. Int J Syst Evol Microbiol 50:993–996 [CrossRef]
    [Google Scholar]
  6. Fiedler F., Kandler O. 1973; Die Mureintypen in der Gattung Cellulomonas Bergey et al . Arch Mikrobiol 89:41–50 (in German [CrossRef]
    [Google Scholar]
  7. Funke G., Ramos C. P., Collins M. D. 1995; Identification of some clinical strains of CDC coryneform group A-3 and A-4 bacteria as Cellulomonas species and proposal of Cellulomonas hominis sp. nov. for some group A-3 strains. J Clin Microbiol 33:2091–2097
    [Google Scholar]
  8. Groth I., Schumann P., Martin K., Schütze B., Augsten K., Kramer I., Stackebrandt E. 1999; Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49:1717–1724 [CrossRef]
    [Google Scholar]
  9. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  10. Rivas R., Trujillo M. E., Mateos P. F., Martínez-Molina E., Velázquez E. 2004; Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree. Int J Syst Evol Microbiol 54:533–536 [CrossRef]
    [Google Scholar]
  11. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  12. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  13. Schumann P., Weiss N., Stackebrandt E. 2001; Reclassification of Cellulomonas cellulans (Stackebrandt and Keddie 1986) as Cellulosimicrobium cellulans gen. nov., comb. nov.. Int J Syst Evol Microbiol 51:1007–1010 [CrossRef]
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Stackebrandt E., Kandler O. 1979; Taxonomy of the genus Cellulomonas , based on phenotypic characters and deoxyribonucleic acid-deoxyribonucleic acid homology, and proposal of seven neotype strains. Int J Syst Bacteriol 29:273–282 [CrossRef]
    [Google Scholar]
  16. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Schumann P., Prauser H. 2002; The family Cellulomonadaceae . In The Prokaryotes , 3rd edn, release 3.10. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H, Stackebrandt E. New York: Springer; http://141.150.157.117 : 8080/prokPUB/chaprender/jsp/showchap.jsp?chapnum=440&initsec=01_
    [Google Scholar]
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