1887

Abstract

A Gram-negative, aerobic, gliding, orange–yellow marine bacterium was isolated from particulate material sampled from the Southern Ocean. This strain produced an exopolysaccharide in liquid culture. 16S rRNA gene sequence analysis showed that this isolate was a member of the family , but represented a separate lineage. Major whole-cell fatty acids included i15 : 110, i15 : 0, -OH i15 : 0, a15 : 110, 15 : 0 and -OH i15 : 0. The G+C content of the DNA was 49 mol%. Based on phylogenetic, phenotypic, chemotaxonomic and genotypic analyses, this bacterium was placed in a novel taxon as gen. nov., sp. nov. with type strain CAM030 (=ACAM 1065=CIP 108537).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63642-0
2005-07-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/4/ijs551557.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63642-0&mimeType=html&fmt=ahah

References

  1. Asper, V. L. & Smith, W. O. ( 2003; ). Abundance, distribution and sinking rates of aggregates in the Ross Sea, Antarctica. Deep Sea Res Part I 50, 131–150.[CrossRef]
    [Google Scholar]
  2. Atlas, R. ( 1993; ). Handbook of Microbiological Media. Boca Raton, FL: CRC Press.
  3. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  4. Bowman, J. P. & Nichols, D. S. ( 2005; ). Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia. Int J Syst Evol Microbiol 55, 1471–1486.[CrossRef]
    [Google Scholar]
  5. Bowman, J. P., Cavanagh, J., Austin, J. J. & Sanderson, K. ( 1996; ). Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46, 841–848.[CrossRef]
    [Google Scholar]
  6. Bowman, J. P., McCammon, S. A., Brown, J. L., Nichols, P. D. & McMeekin, T. A. ( 1997; ). Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47, 670–677.[CrossRef]
    [Google Scholar]
  7. Bowman, J. P., McCammon, S. A., Lewis, T., Skerratt, J. H., Brown, J. L., Nichols, D. S. & McMeekin, T. A. ( 1998; ). Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense gen. nov., comb. nov. Microbiology 144, 1601–1609.[CrossRef]
    [Google Scholar]
  8. Bowman, J. P., Mancuso Nichols, C. & Gibson, J. A. E. ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef]
    [Google Scholar]
  9. Decho, A. W. ( 1990; ). Microbial exopolymer secretions in ocean environments: their role(s) in food webs and marine processes. In Oceanography Marine Biology Annual Review, pp. 73–153. Edited by M. Barnes. Aberdeen: Aberdeen University Press.
  10. Felsenstein, J. ( 1993; ). phylip (Phylogeny Inference Package). Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Flemming, H.-C. & Wingender, J. ( 2001; ). Relevance of microbial extracellular polymeric substances (EPSs). Part I: Structural and ecological aspects. Water Sci Technol 43, 1–8.
    [Google Scholar]
  12. Fowler, S. W. & Knauer, G. A. ( 1986; ). Role of large particles in the transport of elements and organic compounds through the oceanic water column. Prog Oceanogr 16, 147–194.[CrossRef]
    [Google Scholar]
  13. Garrity, G. M. & Holt, J. G. ( 2001; ). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–168. Edited by D. R. Boone, R. W. Castenholtz & G. M. Garrity. New York: Springer.
  14. Ivanova, E. P., Alexeeva, Y. V., Flavier, S., Wright, J. P., Zhukova, N. V., Gorshkova, N. M., Mikhailov, V. V., Nicolau, D. V. & Christen, R. ( 2004; ). Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 54, 705–711.[CrossRef]
    [Google Scholar]
  15. Kiorboe, T. ( 2001; ). Formation and fate of marine snow: small-scale processes with large scale implications. Sci Mar 65, 57–71.
    [Google Scholar]
  16. Kirchman, D. L. ( 2002; ). The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiol Ecol 39, 91–100.
    [Google Scholar]
  17. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  18. Logan, B. E. & Hunt, J. R. ( 1987; ). Advantages to microbes of growth in permeable aggregates in marine systems. Limnol Oceanogr 32, 1034–1048.[CrossRef]
    [Google Scholar]
  19. Mancuso Nichols, C., Garon Lardière, S., Bowman, J. P., Nichols, P. D., Gibson, J. A. E. & Guezennec, J. ( 2005; ). Chemical characterization of exopolysaccharides from Antarctic marine bacteria. Microb Ecol (in press).
    [Google Scholar]
  20. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  21. Mueller-Niklas, G., Schuster, S., Kaltenboeck, E. & Herndl, G. J. ( 1994; ). Organic content and bacterial metabolism in amorphous aggregations of the northern Adriatic Sea. Limnol Oceanogr 39, 58–68.[CrossRef]
    [Google Scholar]
  22. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 7 other authors ( 2004; ). Algibacter lectus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from green algae. Int J Syst Evol Microbiol 54, 1257–1261.[CrossRef]
    [Google Scholar]
  23. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 12 other authors ( 2005a; ). Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 55, 49–55.[CrossRef]
    [Google Scholar]
  24. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Frolova, G. M., Mikhailov, V. V. & Bae, K. S. ( 2005b; ). Bizonia paragorgiae gen. nov., sp. nov., a novel bacterium of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea. Int J Syst Evol Microbiol 55, 375–378.[CrossRef]
    [Google Scholar]
  25. Rue, E. L. & Bruland, K. W. ( 1995; ). Complexation of iron (III) by natural organic ligands in the Central North Pacific as determined by a new competitive ligand equilibrium/adsorptive cathode stripping voltametric method. Mar Chem 50, 117–138.[CrossRef]
    [Google Scholar]
  26. Scharek, R., Vanleeuwe, M. A. & Debaar, H. J. W. ( 1997; ). Responses of Southern Ocean phytoplankton to the addition of trace metals. Deep Sea Res Part II 44, 209–227.[CrossRef]
    [Google Scholar]
  27. Simon, M., Glöckner, F. O. & Amann, R. ( 1999; ). Different community structure and temperature optima of heterotrophic picoplankton in various regions of the Southern Ocean. Aquat Microb Ecol 18, 275–284.[CrossRef]
    [Google Scholar]
  28. Sly, L. I., Blackall, L. L., Kraat, P. C., Tian-Shen, T. & Sangkhobol, V. ( 1986; ). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5, 139–156.[CrossRef]
    [Google Scholar]
  29. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 611–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  30. Sutherland, I. W. ( 2001; ). Biofilm exopolysaccharides: a strong and sticky framework. Microbiology 147, 3–9.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63642-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63642-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 1557 - 1561

Whole-cell fatty acid profiles of CAM030 and related genera in the family . [PDF](27 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error