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A Gram-positive, non-motile actinobacterium, designated strain YIM 70177T, was isolated from a saline soil sample from Qinghai Province, north-west China. Phylogenetic analysis of this organism based on 16S rRNA gene sequences revealed 98·3 % similarity to Isoptericola variabilis DSM 10177T. Chemotaxonomic data determined for the isolate, such as the peptidoglycan type A4α, variation l-lys–d-Asp, supported the placement of strain YIM 70177T within the genus Isoptericola. Galactose was detected as cell wall sugar. Phospholipids identified were phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol, and the predominant menaquinones were MK-9(H4) and MK-9(H2). The major fatty acids were ai-C15 : 0, C16 : 0, ai-C17 : 0 and i-C15 : 0. The DNA G+C content was 72·8 mol%. The low level of DNA–DNA relatedness (27·4 %) to I. variabilis DSM 10177T in combination with differentiating chemotaxonomic and physiological data demonstrated that the isolate YIM 70177T should be classified as representing a novel species of the genus Isoptericola. The name Isoptericola halotolerans sp. nov. is proposed, with strain YIM 70177T (=DSM 16376T=KCTC 19046T) as the type strain.
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