1887

Abstract

Strain DSM 17733, isolated from the shore of Lake Abjata in Ethiopia, is a heterotrophic, alkaliphilic, moderately halophilic, Gram-positive, strictly aerobic, non-motile,non-endospore-forming bacterium. The organism grows optimally at 30–37 °C, pH 9 and 3 % (w/v) NaCl. Analysis of the cell wall showed the presence of murein of the type -lys–gly–-Glu, variation A4. The G+C content of the genomic DNA was 69.0 mol%. Sequence analysis of 16S rRNA gene sequence of strain DSM 17733 placed the isolate in the genus . DNA–DNA hybridization of DSM 17733 with those organisms with the closest phylogenetic affiliation, i.e. , and , gave relatedness values of 48.5 %, 63.7 % (repetition, 57.2 %) and 35.7 % (repetition, 29.3 %), respectively. On the basis of both phenotypic and phylogenetic criteria and the low levels of DNA–DNA relatedness with the phylogenetically closest species and , it is proposed that the isolate be classified in a novel species, sp. nov. The type strain is DSM 17733 (=CCUG 48939).

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2006-06-01
2020-09-22
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References

  1. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Lawson P. A., Labrenz M., Tindall B. J., Weiss N., Hirsch P. 2002; Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia . Int J Syst Evol Microbiol 52:1145–1150 [CrossRef]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. Escara J. F., Hutton J. R. 1980; Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19:1315–1327 [CrossRef]
    [Google Scholar]
  5. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  6. Jahnke K. D. 1992; Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
    [Google Scholar]
  7. Li W. J., Chen H. H., Zhang Y. Q., Schumann P., Stackebrandt E., Xu L. H., Jiang C. L. 2004; Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., two novel actinobacteria from saline soils in the west of China. Int J Syst Evol Microbiol 54:837–841 [CrossRef]
    [Google Scholar]
  8. Li W. J., Chen H. H., Kim C. J., Zhang Y. Q., Park D. J., Lee J. C., Xu L. H., Jiang C. L. 2005; Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia . Int J Syst Evol Microbiol 55:463–466 [CrossRef]
    [Google Scholar]
  9. Logan N. A., Berkeley R. C. W. 1984; Identification of Bacillus strains using the API system. J Gen Microbiol 130:1871–1882
    [Google Scholar]
  10. Maidak B. L., Cole J., Lilburn T. 9 other authors 2000; The RDP (Ribosomal Database Project) continues. Nucleic Acids Res 28:173–174 [CrossRef]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Mota R. R., Marquez C., Arahal D., Mellado E., Ventosa A. 1997; Polyphasic taxonomy of Nesterenkonia halobia . Int J Syst Bacteriol 47:1231–1235 [CrossRef]
    [Google Scholar]
  13. Onishi H., Kamekura M. 1972; Micrococcus halobius sp. nov. Int J Syst Bacteriol 22:233–236 [CrossRef]
    [Google Scholar]
  14. Quillaguamán J., Hatti-Kaul R., Mattiasson B., Alvarez M. T., Delgado O. 2004; Halomonas boliviensis sp. nov., an alkalitolerant, moderate halophile isolated from soil around a Bolivian hypersaline lake. Int J Syst Evol Microbiol 54:721–725 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989; Gel electrophoresis of DNA. In Molecular Cloning: a Laboratory Manual , 2nd edn. pp. 6.3–6.19 Edited by Nolan C. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  18. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  611–651 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. 1995; Taxonomic dissection of the genus Micrococcus : Kocuria gen.nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45:682–692 [CrossRef]
    [Google Scholar]
  21. Tang S. K., Li W., Wang D., Zhang Y. G., Xu L. H., Jiang C. L. 2003; Studies of the biological characteristics of some halophilic and halotolerant actinomycetes isolated from saline and alkaline soils. Actinomycetologica 17:6–10 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
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