1887

Abstract

Three Gram-positive, rod- or coccoid-shaped bacterial strains, KSL-2, KSL-5 and KSL-6, were isolated from an alkaline soil in Korea and subjected to a polyphasic taxonomical analysis. These isolates grew optimally at pH 9·0 and 30 °C. They were characterized chemotaxonomically as having cell wall peptidoglycan based on -2,6-diaminopimelic acid, MK-8(H) as the predominant menaquinone and iso-C as the major fatty acid. The DNA G+C content of the isolates was 73–74 mol%. Strains KSL-2, KSL-5 and KSL-6 were identical in their 16S rRNA gene sequences and exhibited DNA–DNA relatedness values of 88–93 %. Phylogenetic trees based on 16S rRNA gene sequences showed that the three isolates fell within the evolutionary radiation encompassed by the genus . Levels of 16S rRNA gene sequence similarity between the three strains and the type strains of species ranged from 93·6 % (with ) to 97·2 % (with ). DNA–DNA relatedness levels between the three isolates and CJ-14 were 8–15 %. On the basis of phenotypic, phylogenetic and genetic data, strains KSL-2, KSL-5 and KSL-6 were classified in the genus as members of a novel species for which the name sp. nov. is proposed, with KSL-2 (=KCTC 19038=DSM 16314) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63631-0
2005-07-01
2020-11-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/4/ijs551611.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63631-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Dorsch M., Stackebrandt E. 1989; Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to Nocardioides as Nocardioides jensenii comb. nov. Int J Syst Bacteriol 39:1–6 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Cockcroft S., Wallbanks S. 1994; Phylogenetic analysis of a new ll-diaminopimelic acid-containing coryneform bacterium from herbage, Nocardioides plantarum sp. nov. Int J Syst Bacteriol 44:523–526 [CrossRef]
    [Google Scholar]
  3. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  6. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  7. Lawson P. A., Collins M. D., Schumann P., Tindall B. J., Hirsch P., Labrenz M. 2000; New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp.nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23:219–229 [CrossRef]
    [Google Scholar]
  8. Lechevalier M. P., Lechevalier H. A. 1970; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales pp  393–405 Edited by Prauser H. Jena: Gustav Fischer Verlag;
    [Google Scholar]
  9. O'Donnell A. G., Goodfellow M., Minnikin D. E. 1982; Lipids in the classification of Nocardioides : reclassification of Arthrobacter simplex (Jensen) Lochhead in the genus Nocardioides (Prauser) emend.O'Donnell et al . as Nocardioides simplex comb. nov. Arch Microbiol 133:323–329 [CrossRef]
    [Google Scholar]
  10. Prauser H. 1976; Nocardioides , a new genus of the order Actinomycetales . Int J Syst Bacteriol 26:58–65 [CrossRef]
    [Google Scholar]
  11. Prauser H. 1984; Nocardioides luteus spec. nov. Z Allg Mikrobiol 24:647–648 [CrossRef]
    [Google Scholar]
  12. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI Inc;
    [Google Scholar]
  13. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  14. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  15. Suzuki K., Komagata K. 1983; Pimelobacter gen. nov., a new genus of coryneform bacteria with ll-diaminopimelic acid in the cell wall. J Gen Appl Microbiol 29:59–71 [CrossRef]
    [Google Scholar]
  16. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  18. Yi H., Chun J. 2004a; Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:1295–1299 [CrossRef]
    [Google Scholar]
  19. Yi H., Chun J. 2004b; Nocardioides aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:2151–2154 [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  21. Yoon J.-H., Rhee S.-K., Lee J.-S., Park Y.-H., Lee S. T. 1997; Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. Int J Syst Bacteriol 47:933–938 [CrossRef]
    [Google Scholar]
  22. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  23. Yoon J.-H., Cho Y.-G., Lee S. T., Suzuki K.-I., Nakase T., Park Y.-H. 1999; Nocardioides nitrophenolicus sp. nov., a p -nitrophenol-degrading bacterium. Int J Syst Bacteriol 49:675–680 [CrossRef]
    [Google Scholar]
  24. Yoon J.-H., Kim I.-G., Kang K. H., Oh T.-K., Park Y.-H. 2004; Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. Int J Syst Evol Microbiol 54:71–75 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Kim I.-G., Lee M.-H., Lee C.-H., Oh T.-K. 2005; Nocardioides alkalitolerans sp. nov., isolated from an alkaline serpentinite soil in Korea. Int J Syst Evol Microbiol 55:809–814 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63631-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63631-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error