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Volume 55, Issue 5

gen. nov., sp. nov., an aerobic, chitosanase-producing member of the ‘’ Free

Abstract

Four strains (3001, 2, 12 and 13), which were isolated as chitosanase-producing bacteria from soil from Matsue city (Japan), were studied phenotypically, genotypically and phylogenetically. Based on sequence analysis of 16S rRNA genes, DNA G+C content (67·4–69·2 mol%), quinone type (UQ-8), major fatty acid composition (3-OH 10 : 0, 3-OH 14 : 0) and other phylogenetic studies, strains 3001, 12 and 13 were found to occupy a separate position in the ‘’. , and were their closest neighbours (93–95 % 16S rRNA gene sequence similarity). The 16S rRNA gene sequence and other characteristics suggested that strain 2 belonged to the genus . DNA–DNA hybridization experiments supported the conclusion that strains 3001, 12 and 13 were of the same species (72–78 % DNA hybridization) and only distantly related to and . It is proposed that strains 3001, 12 and 13 represent a novel genus and species for which the name gen. nov., sp. nov. is proposed. The type strain of is 3001 (=IAM 14711=ATCC BAA-476).

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Keyword(s): MK, menaquinone and UQ, ubiquinone
SGM
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2005-09-01
2024-04-20
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Mitsuaria chitosanitabida gen. nov., sp. nov., an aerobic, chitosanase-producing member of the ‘Betaproteobacteria’
Int J Syst Evol Microbiol 55, 1927 (2005); https://doi.org/10.1099/ijs.0.63629-0
/content/journal/ijsem/10.1099/ijs.0.63629-0
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