1887

Abstract

Four strains (3001, 2, 12 and 13), which were isolated as chitosanase-producing bacteria from soil from Matsue city (Japan), were studied phenotypically, genotypically and phylogenetically. Based on sequence analysis of 16S rRNA genes, DNA G+C content (67·4–69·2 mol%), quinone type (UQ-8), major fatty acid composition (3-OH 10 : 0, 3-OH 14 : 0) and other phylogenetic studies, strains 3001, 12 and 13 were found to occupy a separate position in the ‘’. , and were their closest neighbours (93–95 % 16S rRNA gene sequence similarity). The 16S rRNA gene sequence and other characteristics suggested that strain 2 belonged to the genus . DNA–DNA hybridization experiments supported the conclusion that strains 3001, 12 and 13 were of the same species (72–78 % DNA hybridization) and only distantly related to and . It is proposed that strains 3001, 12 and 13 represent a novel genus and species for which the name gen. nov., sp. nov. is proposed. The type strain of is 3001 (=IAM 14711=ATCC BAA-476).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63629-0
2005-09-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/5/ijs551927.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63629-0&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  2. Emerson D., Ghiorse W. C. 1993; Role of disulfide bonds in maintaining the structural integrity of the sheath of Leptothrix discophora SP-6. J Bacteriol 175:7819–7827
    [Google Scholar]
  3. Hiraishi A. 1994; Phylogenetic affiliations of Rhodoferax fermentans and related species of phototrophic bacteria as determined by automated 16S rRNA sequencing. Curr Microbiol 28:25–29 [CrossRef]
    [Google Scholar]
  4. Hiraishi A., Hoshino Y., Satoh T. 1991; Rhodoferax fermentans gen. nov. sp. nov. a phototrophic purple nonsulfur bacterium previously referred to as the “ Rhodocyclus gelatinosus -like” group. Arch Microbiol 155330–336
    [Google Scholar]
  5. Hochkoeppler A., Kofod P., Ferro G., Ciurli S. 1995; Isolation, characterization, and functional role of the high-potential iron-sulfur protein (HiPIP) from Rhodoferax fermentans . Arch Biochem Biophys 322:313–318 [CrossRef]
    [Google Scholar]
  6. Kalmbach S., Manz W., Wecke J., Szewzyk U. 1999; Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system. Int J Syst Bacteriol 49:769–777 [CrossRef]
    [Google Scholar]
  7. Maidak B. L., Cole J. R., Parker C. T. Jr 11 other authors 1999; A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  8. Malmqvist A., Welander T., Moore E., Ternstrom A., Molin G., Stenstrom I. M. 1994; Ideonella dechloratans gen. nov., sp. nov. a new bacterium capable of growing anaerobically with chlorate as an electron acceptor. Syst Appl Microbiol 17:58–64 [CrossRef]
    [Google Scholar]
  9. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306 [CrossRef]
    [Google Scholar]
  10. Okada K., Minehira M., Zhu X., Suzuki K., Nakagawa T., Matsuda H., Kawamukai M. 1997; The ispB gene encoding octaprenyl diphosphate synthase is essential for growth of Escherichia coli . J Bacteriol 179:3058–3060
    [Google Scholar]
  11. Park J. K., Shimono K., Ochiai N., Shigeru K., Kurita M., Ohta Y., Tanaka K., Matsuda H., Kawamukai M. 1999; Purification, characterization and gene analysis of a chitosanase (ChoA) from Matsuebacter chitosanotabidus 3001. J Bacteriol 181:6642–6649
    [Google Scholar]
  12. Rogers S. R., Anderson J. J. 1976; Measurement of growth and iron deposition in Sphaerotilus discophorus . J Bacteriol 126:257–263
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [CrossRef]
    [Google Scholar]
  15. Shimono K., Shigeru K., Tsuchiya A., Itou N., Ohta Y., Tanaka K., Nakagawa T., Matsuda H., Kawamukai M. 2002a; Two glutamic acids in chitosanase A from Matsuebacter chitosanotabidus 3001 are the catalytically important residues. J Biochem 131:87–96 [CrossRef]
    [Google Scholar]
  16. Shimono K., Matsuda H., Kawamukai M. 2002b; Functional expression of chitinase and chitosanase, and their effects on morphologies in the yeast Schizosaccharomyces pombe . Biosci Biotechnol Biochem 66:1143–1147 [CrossRef]
    [Google Scholar]
  17. Siering P. L., Ghiorse W. C. 1996; Phylogeny of the Sphaerotilus-Leptothrix group inferred from morphological comparisons, genomic fingerprinting, and 16S ribosomal DNA sequence analyses. Int J Syst Bacteriol 46:173–182 [CrossRef]
    [Google Scholar]
  18. Spring S., Kämpfer P., Ludwig W., Schleifer K. H. 1996; Polyphasic characterization of the genus Leptothrix : new descriptions of Leptothrix mobilis sp. nov. and Leptothrix discophora sp. nov. nom. rev. and emended description of Leptothrix cholodnii emend. Syst Appl Microbiol 19634–643 [CrossRef]
    [Google Scholar]
  19. Stead D. E. 1992; Grouping of plant-pathogenic and some other Pseudomonas spp. by using cellular fatty acid profiles. Int J Syst Bacteriol 42:281–295 [CrossRef]
    [Google Scholar]
  20. Suyama T., Tokiwa Y., Ouichanpagdee P., Kanagawa T., Kamagata Y. 1998; Phylogenetic affiliation of soil bacteria that degrade aliphatic polyesters available commercially as biodegradable plastics. Appl Environ Microbiol 64:5008–5011
    [Google Scholar]
  21. Suyama T., Shigematsu T., Takaichi S., Nodasaka Y., Fujikawa S., Hosoya H., Tokiwa Y., Kanagawa T., Hanada S. 1999; Roseateles depolymerans gen. nov., sp. nov. a new bacteriochlorophyll a -containing obligate aerobe belonging to the β -subclass of the Proteobacteria . Int J Syst Bacteriol 49:449–457 [CrossRef]
    [Google Scholar]
  22. Takeuchi M., Sakane T., Yanagi M., Yamasato K., Hamana K., Yokota A. 1995; Taxonomic study of bacteria isolated from plants: proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp. nov., and Sphingomonas mali sp nov. Int J Syst Bacteriol 45:334–341 [CrossRef]
    [Google Scholar]
  23. Tanasupawat S., Hashimoto Y., Ezaki T., Kozaki M., Komagata K. 1992; Staphylococcus piscifermentans sp. nov., from fermented fish in Thailand. Int J Syst Bacteriol 42:577–581 [CrossRef]
    [Google Scholar]
  24. Willems A., Gillis M., De Ley J. 1991a; Transfer of Rhodocyclus gelatinosus to Rubrivivax gelatinosus gen. nov., comb. nov. and phylogenetic relationships with Leptothrix , Sphaerotilus natans , Pseudomonas saccharophila , and Alcaligenes latus . Int J Syst Bacteriol 41:65–73 [CrossRef]
    [Google Scholar]
  25. Willems A., De Ley J., Gillis M., Kersters K. 1991b; Comamonadaceae , a new family encompassing the acidovorans rRNA complex, including Variovorax paradoxus gen.nov., comb. nov., for Alcaligenes paradoxus (Davis 1969). Int J Syst Bacteriol 41:445–450 [CrossRef]
    [Google Scholar]
  26. Willems A., Goor M., Thielemans S., Gillis M., Kersters K., De Ley J. 1992; Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citrulli Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci . Int J Syst Bacteriol 42:107–119 [CrossRef]
    [Google Scholar]
  27. Yun C.-S., Amakata D., Matsuo Y., Matsuda H., Kawamukai M. 2005; New chitosan-degrading strains that produce chitosanases similar to ChoA of Mitsuaria chitosanitabida . Appl Environ Microbiol (in press
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63629-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63629-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error