1887

Abstract

A novel Gram-positive, aerobic, catalase-negative, coccus-shaped organism originating from tobacco was characterized using phenotypic and molecular taxonomic methods. The organism contained a cell wall murein based on -lysine (variation A4, type -lysine–-glutamic acid), synthesized long-chain cellular fatty acids of the straight-chain saturated and monounsaturated types (with C 9, C and C 9 predominating) and possessed a DNA G+C content of 46 mol%. Based on morphological, biochemical and chemical characteristics, the coccus-shaped organism did not conform to any presently recognized taxon. Comparative 16S rRNA gene sequencing studies confirmed the distinctiveness of the unknown coccus, with the bacterium displaying sequence divergence values of greater than 7 % with other recognized Gram-positive taxa. Treeing analysis reinforced its distinctiveness, with the unidentified organism forming a relatively long subline branching at the periphery of an rRNA gene sequence cluster which encompasses the genera s, , , , and . Based on phenotypic and molecular phylogenetic evidence, it is proposed that the unknown organism from tobacco be classified as a new genus and species, gen. nov., sp. nov. The type strain of is CCUG 48253 (=CIP 108502).

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2005-07-01
2024-03-28
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References

  1. Aguirre M., Collins M. D. 1992; Phylogenetic analysis of Alloiococcus otitis gen. nov., sp. nov. an organism from human middle ear fluid. Int J Syst Bacteriol 42:79–83 [CrossRef]
    [Google Scholar]
  2. Aguirre M., Morrison D., Cookson B. D., Gay F. W., Collins M. D. 1993; Phenotypic and phylogenetic characterization of some Gemella -like organisms from human infections: description of Dolosigranulum pigrum gen. nov., sp. nov. J Appl Bacteriol 75:608–612 [CrossRef]
    [Google Scholar]
  3. Collins M. D. 1994; Isoprenoid quinones . In: Chemical Methods in Prokaryotic Systematics pp  265–309 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  5. Collins M. D., Aguirre M., Facklam R. R., Shallcross J., Williams A. M. 1992; Globicatella sanguis gen. nov., sp. nov., a new gram-positive catalase-negative bacterium from human sources. J Appl Bacteriol 73:433–437 [CrossRef]
    [Google Scholar]
  6. Collins M. D., Falsen E., Lemozy J., Åkervall E., Sjödén B., Lawson P. A. 1997; Phenotypic and phylogenetic characterization of some Globicatella -like organisms from human sources: description of Facklamia hominis gen. nov., sp. nov. Int J Syst Bacteriol 47:880–882 [CrossRef]
    [Google Scholar]
  7. Collins M. D., Lawson P. A., Monasterio R., Felsen E., Sjödén B., Facklam R. R. 1999a; Ignavigranum ruoffiae gen. nov., sp. nov., isolated from human clinical specimens. J Clin Microbiol 37:1161–1164
    [Google Scholar]
  8. Collins M. D., Rodriguez Jovita M., Hutson R. A., Falsen E., Sjödén B., Facklam R. R. 1999b; Dolosicoccus paucivorans gen. nov., sp. nov., isolated from human blood. Int J Syst Bacteriol 49, 1439–1442 [CrossRef]
  9. Collins M. D., Higgins R., Messier S., Fortin M., Hutson R. A., Lawson P. A., Falsen E. 2003; Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative rod-shaped bacterium from pig semen. Int J Syst Evol Microbiol 53:811–814 [CrossRef]
    [Google Scholar]
  10. Facklam R. R., Elliott J. A. 1995; Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev 8:479–495
    [Google Scholar]
  11. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov. a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [CrossRef]
    [Google Scholar]
  12. Ishikawa M., Nakajima K., Yanagi M., Yamamoto Y., Yamasato K. 2003; Marinilactobacillus psychrotolerans gen. nov. sp. nov. a halophilic and alkaliphilic marine lactic acid bacterium isolated from marine organisms in temperate and subtropical areas of Japan. Int J Syst Evol Mcrobiol 53711–720 [CrossRef]
    [Google Scholar]
  13. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441 [CrossRef]
    [Google Scholar]
  14. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N -heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Miller P. H., Facklam R. R., Miller J. M. 1996; Atmospheric growth requirements of Alloiococcus species and related gram-positive cocci. J Clin Microbiol 34:1027–1028
    [Google Scholar]
  17. Nicholas K. B., Nicholas H. B. Jr, Deerfield D. W. II 1997; GeneDoc, analysis and visualization of genetic variation. EMBNEW News 4:14
    [Google Scholar]
  18. Page R. D. 1996; treeview, an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  19. Rasmussen S. W. 2002; SEQtools, a software package for analysis of nucleotide and protein sequences. http://www.seqtools.dk
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  22. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
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