1887

Abstract

A moderately halophilic, Gram-negative bacterium, strain BH539, which was isolated from a solar saltern at Taean in Korea, was considered to be a member of the genus . Strain BH539 grew at salinities of 1–25 % (w/v) and at temperatures of 10–45 °C. Cells were short rods that were motile by means of several flagella. Their major fatty acids were C 7, C and C cyclo 8. The genomic DNA G+C content was about 65 mol% and the predominant ubiquinone was Q-9. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate formed a branch of the species . However, 23S and 16S rRNA gene sequence similarities revealed that strain BH539 was related more closely to the type strains of the genus . Phylogenetic analyses based on 23S rRNA gene sequences also indicated that the strain formed a phyletic line within the genus . Therefore, it was concluded that strain BH539 should be classified within the genus , rather than . On the basis of physiological and molecular properties, strain BH539 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BH539 (=KCTC 12284=DSM 16463).

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2005-09-01
2021-04-23
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References

  1. Arahal D. R., García M. T., Ludwig W., Schleifer K. H., Ventosa A. 2001a; Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51:1443–1448
    [Google Scholar]
  2. Arahal D. R., García M. T., Vargas C., Cánovas D., Nieto J. J., Ventosa A. 2001b; Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. Int J Syst Evol Microbiol 51:1457–1462
    [Google Scholar]
  3. Arahal D. R., Castillo A. M., Ludwig W., Schleifer K. H., Ventosa A. 2002a; Proposal of Cobetia marina gen. nov., comb. nov. within the family Halomonadaceae , to include the species Halomonas marina . Syst Appl Microbiol 25:207–211 [CrossRef]
    [Google Scholar]
  4. Arahal D. R., Ludwig W., Schleifer K. H., Ventosa A. 2002b; Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Int J Syst Evol Microbiol 52:241–249
    [Google Scholar]
  5. Baumann L., Bowditch R. D., Baumann P. 1983; Description of Deleya gen. nov. created to accommodate the marine species Alcaligenes aestus , A. pacificus , A. cupidus , A. venustus , and Pseudomonas marina . Int J Syst Bacteriol 33:793–802 [CrossRef]
    [Google Scholar]
  6. Bouchotroch S., Quesada E., del Moral A., Llamas I., Béjar V. 2001; Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 51:1625–1632 [CrossRef]
    [Google Scholar]
  7. Cole J. R., Chai B., Marsh T. L. 8 other authors 2003; The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
    [Google Scholar]
  8. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  9. DeLong E. F. 1992; Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89:5685–5689 [CrossRef]
    [Google Scholar]
  10. Dobson S. J., Franzmann P. D. 1996; Unification of the genera Deleya (Baumann et al . 1983), Halomonas (Vreeland et al . 1980),and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas , and placement of the genus Zymobacter in the family Halomonadaceae . Int J Syst Bacteriol 46:550–558 [CrossRef]
    [Google Scholar]
  11. Felsenstein F. 2002 phylip (Phylogeny Inference Package) version 3.6a Seattle: University of Washington;
    [Google Scholar]
  12. García M. T., Mellado E., Ostos J. C., Ventosa A. 2004; Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. Int J Syst Evol Microbiol 54:1723–1728 [CrossRef]
    [Google Scholar]
  13. Garriga M., Ehrmann M. A., Arnau J., Hugas M., Vogel R. F. 1998; Carnimonas nigrificans gen. nov., sp. nov. a bacterial causative agent for black spot formation on cured meat products. Int J Syst Bacteriol 48:677–686 [CrossRef]
    [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  15. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–206
    [Google Scholar]
  16. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  17. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  18. Lim J.-M., Yoon J.-H., Lee J.-C., Jeon C. O., Park D.-J., Sung C., Kim C.-J. 2004; Halomonas koreensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 54:2037–2042 [CrossRef]
    [Google Scholar]
  19. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. 2002; A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [CrossRef]
    [Google Scholar]
  20. Mellado E., Moore E. R. B., Nieto J. J., Ventosa A. 1995; Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui , Volcaniella eurihalina , and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45:712–716 [CrossRef]
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  24. Valderrama M. J., Monteoliva-Sanchez M., Quesada E., Ramos-Cormenzana A. 1998; Influence of salt concentration on the cellular fatty acid composition of the moderately halophilic bacterium Halomonas salina . Res Microbiol 149:675–679 [CrossRef]
    [Google Scholar]
  25. Ventosa A., Nieto J. J., Oren A. 1998; Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62:504–544
    [Google Scholar]
  26. Vreeland R. H., Martin E. L. 1980; Growth characteristics, effects of temperature, and ion specificity of the halotolerant bacterium Halomonas elongata . Can J Microbiol 26:746–752 [CrossRef]
    [Google Scholar]
  27. Yakimov M. M., Golyshin P. N., Lang S., Moore E. R. B., Abraham W.-R., Lünsdorf H., Timmis K. N. 1998; Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48:339–348 [CrossRef]
    [Google Scholar]
  28. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  29. Yoon J.-H., Lee K.-C., Kho Y. H., Kang K. H., Kim C.-J., Park Y.-H. 2002; Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52:123–130
    [Google Scholar]
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