1887

Abstract

Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys ( L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and gene sequencing, and from the most closely related species by using DNA–DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the species group (99 % similarity) but are biochemically similar to , from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from -mannitol, the inability to hydrolyse aesculin and DNA and the absence of -glucosidase. On the basis of these analyses, a novel species of the genus is described, for which the name sp. nov. is proposed, with CCM 7213 (=LMG 22723) as the type strain.

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2005-09-01
2019-10-18
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vol. , part 5, pp. 1953 - 1958

Dendrogram based on cluster analysis of the SDS-PAGE profiles.

Dendrogram based on cluster analysis of RI ribotypes, and schematic representation of ribotype patterns for sp. nov. and selected species.

16S–23S rRNA intergenic transcribed spacer-PCR patterns of sp. nov. and selected control strains.

PFGE separation of DNA from sp. nov. digested with I restriction endonuclease.

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