1887

Abstract

A novel species of the genus was isolated from sediments collected on the deep-sea floor in the north-west Pacific Ocean. Strains SY-96, isolated from the Yap Trench, and SY-246, isolated from the Iheya Ridge, had almost identical nucleotide sequences for their internal transcribed spacers and their 5·8S rDNA. Their physiological characteristics were also almost identical. The strains were assumed to be related to and based on sequence similarities in the D1/D2 region of the 26S rDNA. The low DNA–DNA relatedness and sequence similarity between strain SY-96 and related species revealed that strains SY-96 and SY-246 represent a hitherto unknown species. As ballistoconidia and sexual reproduction were not observed in strains SY-96 and SY-246, these strains are described as sp. nov. The type strain is SY-96 (=JCM 10908=CBS 10070).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63584-0
2006-01-01
2019-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/1/295.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63584-0&mimeType=html&fmt=ahah

References

  1. Bai, F.-Y., Takashima, M. & Nakase, T. ( 2001a; ). Description of Bullera kunmingensis sp. nov., and clarification of the taxonomic status of Bullera sinensis and its synonyms based on molecular phylogenetic analysis. FEMS Yeast Res 1, 103–109.[CrossRef]
    [Google Scholar]
  2. Bai, F.-Y., Takashima, M. & Nakase, T. ( 2001b; ). Phylogenetic analysis of strains originally assigned to Bullera variabilis: descriptions of Bullera pseudohuiaensis sp. nov., Bullera komagatae sp. nov. and Bullera pseudoschimicola sp. nov. Int J Syst Evol Microbiol 51, 2177–2187.[CrossRef]
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Fell, J. W., Boekhout, T., Fonseca, A., Scorzetti, G. & Statzell-Tallman, A. ( 2000; ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50, 1351–1371.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Fonseca, Á., Scorzetti, G. & Fell, J. W. ( 2000; ). Diversity in the yeast Cryptococcus albidus and related species as revealed by ribosomal DNA sequence analysis. Can J Microbiol 46, 7–27.[CrossRef]
    [Google Scholar]
  8. Gadanho, M. & Sampaio, J. P. ( 2002; ). Polyphasic taxonomy of the basidiomycetous yeast genus Rhodotorula: Rh. glutinis sensu stricto and Rh. dairenensis comb. nov. FEMS Yeast Res 2, 47–58.
    [Google Scholar]
  9. Hamamoto, M. & Nakase, T. ( 1995; ). Ballistosporous yeasts found on the surface of plant materials collected in New Zealand. 1. Six new species in the genus Sporobolomyces. Antonie van Leeuwenhoek 67, 151–171.[CrossRef]
    [Google Scholar]
  10. Hamamoto, M., Sugiyama, J. & Komagata, K. ( 1987; ). DNA-DNA reassociation studies of strains in the genera Rhodosporidium and Rhodotorula. J Gen Appl Microbiol 33, 57–73.[CrossRef]
    [Google Scholar]
  11. Hamamoto, M., Nagahama, T. & Tamura, M. ( 2002; ). Systematic study of basidiomycetous yeasts–evaluation of the ITS regions of rDNA to delimit species of the genus Rhodosporidium. FEMS Yeast Res 2, 409–413.
    [Google Scholar]
  12. Komagata, K. & Nakase, T. ( 1967; ). Reitoshokuhin no biseibutsu ni kansuru kenkyu. V. Shihan reitoshokuhin yori bunri shita kobo no seijo (Microbiological studies on frozen foods). Shokuhin Eiseigaku Zasshi 8, 53–57 (in Japanese).[CrossRef]
    [Google Scholar]
  13. Nagahama, T., Hamamoto, M., Nakase, T., Takami, H. & Horikoshi, K. ( 2001a; ). Distribution and identification of red yeasts in deep-sea environments around the northwest Pacific Ocean. Antonie van Leeuwenhoek 80, 101–110.[CrossRef]
    [Google Scholar]
  14. Nagahama, T., Hamamoto, M., Nakase, T. & Horikoshi, K. ( 2001b; ). Rhodotorula lamellibrachii sp. nov., a new yeast species from a tubeworm collected at the deep-sea floor in Sagami Bay and its phylogenetic analysis. Antonie van Leeuwenhoek 80, 317–323.[CrossRef]
    [Google Scholar]
  15. Nagahama, T., Hamamoto, M., Nakase, T. & Horikoshi, K. ( 2003; ). Rhodotorula benthica sp. nov. and Rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and Rhodotorula lysiniphila sp. nov., which is related phylogenetically. Int J Syst Evol Microbiol 53, 897–903.[CrossRef]
    [Google Scholar]
  16. Nakase, T. & Suzuki, M. ( 1986; ). Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32, 225–240.[CrossRef]
    [Google Scholar]
  17. Posada, D. & Crandall, K. A. ( 1998; ). modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.[CrossRef]
    [Google Scholar]
  18. Rambaut, A. ( 1996; ). Se-Al: Sequence Alignment Editor version 1.0α1. Distributed by the author. Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 4JD, UK. http://evolve.zoo.ox.ac.uk/
  19. Swofford, D. L. ( 2003; ). paup* 4.0: Phylogenetic analysis using parsimony (*and other methods). Sunderland, MA: Sinauer Associates.
  20. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignments through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. White, T. J., Bruns, T., Lee, S. & Taylor, J. W. ( 1990; ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols, pp. 315–322. Edited by M. Innis, D. Gelfand, J. J. Sninsky & T. J. White. San Diego, CA: Academic Press.
  23. Yamada, Y. & Kondo, K. ( 1973; ). Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19, 59–77.[CrossRef]
    [Google Scholar]
  24. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63584-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63584-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error