sp. nov., isolated from an alkaline soil, and emended description of the genus Free

Abstract

A Gram-positive, aerobic, non-motile bacterial strain, KSL-107, was isolated from an alkaline soil from Korea and subjected to a polyphasic taxonomic study. Strain KSL-107 grew optimally at 25 °C and pH 7·0–7·5. Strain KSL-107 had a cell-wall peptidoglycan based on -2,6-diaminopimelic acid. It contained MK-9(H) as the predominant menaquinone and C, C 2-OH and 10-methyl C as the major fatty acids. The DNA G+C content was 71·5 mol%. Comparative 16S rRNA gene sequence analyses showed that strain KSL-107 was phylogenetically affiliated to the genus of the family . Similarity values between the 16S rRNA gene sequence of strain KSL-107 and those of type strains of species were in the range 97·9–98·2 %. The isolate could be distinguished from other species by levels of DNA–DNA relatedness and differences in some phenotypic characteristics. On the basis of the data presented, it is suggested that strain KSL-107 (=KCTC 19073=DSM 16824) represents a novel species, for which the name sp. nov. is proposed. An emended description of the genus is also proposed.

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2005-09-01
2024-03-28
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