1887

Abstract

A halophilic, alkalitolerant bacterium, strain 20AG, was isolated from an algal mat collected from a sulfurous spring located in Santa Maria Incaldana (Mondragone, Campania Region, southern Italy). The isolate is Gram-positive, ferments several carbohydrates and has motile, rod-shaped cells that do not sporulate. The isolate grows at pH 6·5–9·5 and in 5–20 % NaCl. On the basis of 16S rRNA gene sequence similarity, the strain was shown to belong to the genus ; strain 20AG showed 96·6 % 16S rRNA gene sequence similarity to the type strain of , DSM 14371, and 99·5 % similarity to NCIMB 14022. Levels of DNA–DNA relatedness between strain 20AG and DSM 14371 and NCIMB 14022 were respectively 29·4 and 59·0 %. The G+C content of the DNA of strain 20AG was 40·1 mol%. The predominant respiratory quinone was MK-7, phosphatidylglycerol and phosphatidylcholine were the predominant polar lipids and minor phospholipids were also detected. ai-C14 : 0, ai-C15 : 0 and i-C15 : 0 were the major fatty acids. Strain 20AG accumulated osmolytes and produced exopolysaccharide. On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, isolate 20AG should be designated as the type strain of a subspecies of , for which the name subsp subsp. nov. is proposed. The type strain is 20AG (=DSM 16557=ATCC BAA-954).

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2006-04-01
2020-01-22
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