1887

Abstract

Twenty-one strains of a novel ascomycetous yeast species were isolated from soil collected in three kinds of natural forest, namely a dry dipterocarp forest, a mixed deciduous forest and a pine forest, in Nam Nao National Park, Phetchabun province, Thailand. The strains formed asci containing one to four ovoid to reniform ascospores, assimilated glucose, galactose and glycerol, fermented glucose and galactose vigorously and contained ubiquinone Q-6, indicating that they belonged to the genus . A comparative analysis of the small subunit rDNA (SSU rDNA) and the D1/D2 domain of the large subunit rDNA (LSU rDNA) of all available sequences for ascomycetous yeasts confirmed that the strains were phylogenetically related to the genus . All strains had identical nucleotide sequences in the D1/D2 domain of the LSU rDNA and differed from the nearest species, IFO 10925, by 6·4 % nucleotide substitutions. The strains differed from by the ability to assimilate -gluconic acid, the inability to grow on 50 % glucose medium, the nuclear DNA base composition and deliquescent asci. The strains were differentiated from the other four species of on the basis of trehalose assimilation, the ability to grow on 50 % glucose or 10 % NaCl plus 5 % glucose, vitamin requirement, the nuclear DNA base composition and the type of ascus. Based on the characteristics mentioned above, the strains are recognized as a single novel species of the genus and the name sp. nov. is proposed. The type strain is TN1-01 (=TISTR 5828=JCM 12664=CBS 10093).

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2005-07-01
2020-01-27
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References

  1. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  2. Hamamoto, M. & Nakase, T. ( 1995; ). Ballistosporous yeasts found on the surface of plant materials collected in New Zealand. 1. Six new species in the genus Sporobolomyces. Antonie van Leeuwenhoek 67, 151–171.[CrossRef]
    [Google Scholar]
  3. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  4. Kuraishi, H., Katayama-Fujimura, Y., Sugiyama, J. & Yokoyama, T. ( 1985; ). Ubiquinone systems in fungi. I. Distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic applications. Trans Mycol Soc Jpn 26, 383–395.
    [Google Scholar]
  5. Kurtzman, C. P. ( 2003; ). Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. FEMS Yeast Res 4, 233–245.[CrossRef]
    [Google Scholar]
  6. Kurtzman, C. P. & Robnett, C. J. ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef]
    [Google Scholar]
  7. Lachance, M. A. ( 1998; ). Kluyveromyces van der Walt emend. van der Walt. In The Yeasts, a Taxonomic Study, 4th edn, pp. 227–247. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  8. Lachance, M. A., Bowles, J. M., Starmer, W. T. & Barker, S. F. ( 1999; ). Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 45, 172–177.[CrossRef]
    [Google Scholar]
  9. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  10. Nakase, T. & Suzuki, M. ( 1986; ). Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis, and Sasa sp. in Japan. J Gen Appl Microbiol 32, 225–240.[CrossRef]
    [Google Scholar]
  11. O'Donnell, K. ( 1993; ). Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics, pp. 225–233. Edited by D. R. Reynolds & J. W. Taylor. Wallingford, UK: CAB International.
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Sjamsuridzal, W., Tajiri, Y., Nishida, H., Thuan, T. B., Kawasaki, H., Hirata, A., Yokota, A. & Sugiyama, J. ( 1997; ). Evolutionary relationships of members of the genera Taphrina, Protomyces, Schizosaccharomyces, and related taxa within the Archiascomycetes: integrated analysis of genotypic and phenotypic characters. Mycoscience 38, 267–280.[CrossRef]
    [Google Scholar]
  14. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  15. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  16. Ueda-Nishimura, K. & Mikata, K. ( 1999; ). A new yeast genus, Tetrapisispora gen. nov.: Tetrapisispora iriomotensis sp. nov., Tetrapisispora nanseiensis sp. nov. and Tetrapisispora arboricola sp. nov., from the Nansei Islands, and reclassification of Kluyveromyces phaffii (van der Walt) van der Walt as Tetrapisispora phaffii comb. nov. Int J Syst Bacteriol 49, 1915–1924.[CrossRef]
    [Google Scholar]
  17. van der Walt, J. P. ( 1971; ). New combinations in the genera Brettanomyces, Kluyveromyces, Lodderomyces, and Wingea. Bothalia 10, 417–418.
    [Google Scholar]
  18. Yamada, Y. & Kondo, K. ( 1973; ). Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19, 59–77.[CrossRef]
    [Google Scholar]
  19. Yamada, Y., Kawasaki, H., Nagatsuka, Y., Mikata, K. & Seki, T. ( 1999; ). The phylogeny of the cactophilic yeasts based on the 18S ribosomal RNA gene sequences: the proposals of Phaffomyces antillensis and Starmera caribaea, new combinations. Biosci Biotechnol Biochem 63, 827–832.[CrossRef]
    [Google Scholar]
  20. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
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