1887

Abstract

A new bacterial strain, designated SHRIMP, isolated from the haemolymph of the Pacific white shrimp, , was serologically distinct from other spiroplasmas. Cells of this strain were helical in form and variable in length. Examination by electron microscopy revealed wall-less cells delineated by a single cytoplasmic membrane. The organisms grew well in M1D media supplemented with 2 % NaCl. Strain SHRIMP grew at temperatures of 20–37 °C, with optimum growth occurring at 28 °C. The strain catabolized glucose and hydrolysed arginine, but did not hydrolyse urea. The G+C content of the DNA was 29±1 mol%. Strain SHRIMP (=ATCC BAA-1082=CAIM 1252) is designated the type strain of a novel species, sp. nov., which represents a new subgroup (I-9) of the group I spiroplasmas.

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2005-11-01
2019-10-19
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References

  1. Aluotto, B. B., Wittler, R. G., Williams, C. O. & Faber, J. E. ( 1970; ). Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int J Syst Bacteriol 20, 35–58.[CrossRef]
    [Google Scholar]
  2. Bell, T. A. & Lightner, D. V. ( 1988; ). A Handbook of Normal Penaeid Shrimp Histology, p. 6. Baton Rouge, LA: World Aquaculture Society.
  3. Carle, P., Saillard, C. & Bové, J. M. ( 1983; ). Determination of guanine plus cytosine content of DNA. Methods Mycoplasmol 1, 301–308.
    [Google Scholar]
  4. Clark, T. B. ( 1982; ). Spiroplasmas: diversity of arthropod reservoirs and host–parasite relationships. Science 217, 57–59.[CrossRef]
    [Google Scholar]
  5. Daniels, M. J., Longland, J. M. & Gilbart, J. ( 1980; ). Aspects of motility and chemotaxis in spiroplasmas. J Gen Microbiol 118, 429–436.
    [Google Scholar]
  6. Davis, R. E. & Worley, J. F. ( 1973; ). Spiroplasma: motile, helical microorganism associated with corn stunt disease. Phytopathology 63, 403–408.[CrossRef]
    [Google Scholar]
  7. FAO (Food & Agriculture Organization of the United Nations) ( 2002; ). The State of World Fisheries and Aquaculture (SOFIA). Rome: FAO.
  8. Gasparich, G. E., Hackett, K. J., Stamburski, C., Renaudin, J. & Bové, J. M. ( 1993; ). Optimization of methods for transfecting Spiroplasma citri strain R8A2 HP with the spiroplasma virus SpV1 replicative form. Plasmid 29, 193–205.[CrossRef]
    [Google Scholar]
  9. Gasparich, G. E., Whitcomb, R. F., Dodge, D., French, F. E., Glass, J. & Williamson, D. L. ( 2004; ). The genus Spiroplasma and its non-helical descendants: phylogenetic classification, correlation with phenotype and roots of the Mycoplasma mycoides clade. Int J Syst Evol Microbiol 54, 893–918.[CrossRef]
    [Google Scholar]
  10. Hackett, K. J., Whitcomb, R. F., Tully, J. G. & 9 other authors ( 1993; ). Spiroplasma insolitum sp. nov., a new species of group I spiroplasma with an unusual DNA base composition. Int J Syst Bacteriol 43, 272–277.[CrossRef]
    [Google Scholar]
  11. Holthuis, L. B. ( 1980; ). Shrimp and Prawn of the World (FAO species catalog, vol. 1). FAO Fish Synop. 125. Rome: FAO.
  12. ICSB (International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes) ( 1995; ). Revised minimum standards for description of new species of the class Mollicutes (division Tenericutes). Int J Syst Bacteriol 45, 605–612.[CrossRef]
    [Google Scholar]
  13. Jones, A. L., Whitcomb, R. F., Williamson, D. L. & Coan, M. E. ( 1977; ). Comparative growth and primary isolation of spiroplasmas in media based on insect tissue culture formulations. Phytopathology 67, 738–746.
    [Google Scholar]
  14. Kautsky, N., Ronnback, P., Tedengren, M. & Troell, M. ( 2000; ). Ecosystem perspectives on management of disease in shrimp pond farming. Aquaculture 191, 145–161.[CrossRef]
    [Google Scholar]
  15. Konai, M., Clark, E. A., Camp, M., Koeh, A. L. & Whitcomb, R. F. ( 1996; ). Temperature ranges, growth optima, and growth rates of Spiroplasma (Spiroplasmataceae, class Mollicutes) species. Curr Microbiol 32, 314–319.[CrossRef]
    [Google Scholar]
  16. Lightner, D. V. ( 1993; ). Diseases of cultured penaeid shrimp. In CRC Handbook of Mariculture: Crustacean Aquaculture, 2nd edn, vol. 1, pp. 393–486. Edited by J. P. McVey. Boca Raton, FL: CRC Press.
  17. Lightner, D. V. ( 1996; ). A Handbook of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultured Penaeid Shrimp (Special Publication of the World Aquaculture Society). Baton Rouge, LA: World Aquaculture Society.
  18. Lightner, D. V., Bell, T. A., Redman, R. M., Mohney, L. L., Natividad, J. M., Rukyani, A. & Poernomo, A. ( 1992; ). A review of some major diseases of economic significance in penaeid prawns/shrimps of the Americas and Indopacific. In Diseases in Asian Aquaculture, pp. 57–80. Edited by I. M. Shariff, R. P. Subasinghe & J. R. Arthur. Manila, Philippines: Fish Health Section, Asian Fisheries Society.
  19. Maddison, W. P. & Maddison, D. R. ( 1992; ). macclade: Analysis of Phylogeny and Character Evolution, version 3.0. Sunderland, MA: Sinauer Associates.
  20. Meyer, F. P. ( 1991; ). Aquaculture disease and health management. J Anim Sci 69, 4201–4208.
    [Google Scholar]
  21. Nunan, L. M., Poulos, B., Redman, R., Le Groumellec, M. & Lightner, D. V. ( 2003; ). Molecular detection methods developed for a systemic rickettsia-like bacterium (RLB) in Penaeus monodon (Decapoda: Crustacea). Dis Aquat Organ 53, 15–23.[CrossRef]
    [Google Scholar]
  22. Nunan, L. M., Pantoja, C. R., Salazar, M., Aranguren, F. & Lightner, D. V. ( 2004; ). Characterization and molecular methods for detection of a novel spiroplasma pathogenic to Penaeus vannamei. Dis Aquat Organ 62, 255–264.[CrossRef]
    [Google Scholar]
  23. Pruder, G. D., Brown, C. L., Sweeney, J. M. & Carr, W. H. ( 1995; ). High health shrimp systems: seed supply – theory and practice. In Swimming Through Troubled Water: Proceedings of the Special Session on Shrimp Farming, Aquaculture '95, pp. 40–52. Edited by C. L. Browdy & J. S. Hopkins. Baton Rouge, LA: World Aquaculture Society.
  24. Razin, S., Hasin, M., Ne'eman, Z. & Rottem, S. ( 1973; ). Isolation, chemical composition, and ultrastructural features of the cell membrane of the mycoplasma-like organism Spiroplasma citri. J Bacteriol 116, 1421–1435.
    [Google Scholar]
  25. Swofford, D. L. ( 1998; ). paup*: Phylogenetic Analysis Using Parsimony (*and Other Methods), version 4. Sunderland, MA: Sinauer Associates.
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  27. Trachtenberg, S. ( 1998; ). Mollicutes – wall-less bacteria with internal cytoskeletons. J Struct Biol 124, 244–256.[CrossRef]
    [Google Scholar]
  28. Tully, J. G. ( 1983; ). Cloning and filtration techniques for mycoplasmas. Methods Mycoplasmol 1, 173–177.
    [Google Scholar]
  29. Tully, J. G. & Whitcomb, R. F. ( 1990; ). The genus Spiroplasma. In The Prokarotes, pp. 1960–1980. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. New York: Springer.
  30. Tully, J. G., Whitcomb, R. F., Rose, D. L. & Bové, J. M. ( 1982; ). Spiroplasma mirum, a new species from the rabbit tick (Haemaphysalis leporispalustris). Int J Syst Bacteriol 32, 92–100.[CrossRef]
    [Google Scholar]
  31. Tully, J. G., Bové, J. M., Laigret, F. & Whitcomb, R. F. ( 1993; ). Revised taxonomy of the class Mollicutes: proposed elevation of a monophyletic cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasmatales ord. nov.), with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae fam. nov.) from helical species (Spiroplasmataceae), and emended descriptions of the order Mycoplasmatales, family Mycoplasmataceae. Int J Syst Bacteriol 43, 378–385.[CrossRef]
    [Google Scholar]
  32. Wang, W., Rong, L., Gu, W., Du, K. & Chen, J. ( 2003; ). Study on experimental infections of Spiroplasma from the Chinese mitten crab in crayfish, mice and embryonated chickens. Res Microbiol 154, 677–680.[CrossRef]
    [Google Scholar]
  33. Wang, W., Chen, J., Du, K. & Xu, Z. ( 2004; ). Morphology of spiroplasmas in the Chinese mitten crab Eriocheir sinensis associated with tremor disease. Res Microbiol 155, 630–635.[CrossRef]
    [Google Scholar]
  34. Whitcomb, R. F. ( 1983; ). Culture media for spiroplasmas. Methods Mycoplasmol 1, 147–158.
    [Google Scholar]
  35. Whitcomb, R. F., Tully, J. G., Rose, D. L., Stephens, E. B., Smith, A., McCoy, R. E. & Barile, M. F. ( 1982; ). Wall-less prokaryotes from fall flowers in central United States and Maryland. Curr Microbiol 7, 285–290.[CrossRef]
    [Google Scholar]
  36. Williamson, D. L., Whitcomb, R. F. & Tully, J. G. ( 1978; ). The spiroplasma deformation test, a new serological method. Curr Microbiol 1, 203–207.[CrossRef]
    [Google Scholar]
  37. Williamson, D. L., Tully, J. G. & Whitcomb, R. F. ( 1979; ). Serological relationships of spiroplasmas as shown by combined deformation and metabolism inhibition tests. Int J Syst Bacteriol 29, 345–351.[CrossRef]
    [Google Scholar]
  38. Williamson, D. L., Tully, J. G. & Whitcomb, R. F. ( 1989; ). The genus Spiroplasma. In The Mycoplasmas, vol. 5, pp. 71–111. Edited by R. F. Whitcomb & J. G. Tully. San Diego, CA: Academic Press.
  39. Williamson, D. L., Whitcomb, R. F., Tully, J. G. & 10 other authors ( 1998; ). Revised group classification of the genus Spiroplasma. Int J Syst Bacteriol 48, 1–12.[CrossRef]
    [Google Scholar]
  40. Wyban, J. A., Swingle, J. S., Sweeney, J. N. & Pruder, G. D. ( 1992; ). Development and commercial performance of high health shrimp using specific pathogen free (SPF) broodstock Penaeus vannamei. In Proceedings of the Special Session on Shrimp Farming, pp. 254–259. Edited by J. A. Wyban. Baton Rouge, LA: World Aquaculture Society.
  41. Zbinden, M. & Cambon-Bonavita, M.-A. ( 2003; ). Occurrence of Deferribacterales and Entomoplasmatales in the deep-sea alvinocarid shrimp Rimicaris exoculata gut. FEMS Microbiol Ecol 46, 23–30.[CrossRef]
    [Google Scholar]
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