1887

Abstract

The nucleotide sequences (604 bp) of partial heat-shock protein genes () from 161 strains containing 56 reference species and 105 clinical isolates were determined and compared. sequence analysis showed a higher degree of divergence between species than did 16S rRNA gene analysis. Generally, the topology of the phylogenetic tree based on the DNA sequences was similar to that of the 16S rRNA gene, thus revealing natural relationships among species. When a direct sequencing protocol targeting 422 bp sequences was applied to 70 non-tuberculous mycobacterium (NTM) clinical isolates, all NTMs were clearly identified. In addition, an I PCR restriction fragment length polymorphism analysis method for the differentiation of complex from NTM strains was developed during this study. The results obtained suggest that 604 bp sequences are useful for the phylogenetic analysis and species identification of mycobacteria.

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2005-07-01
2019-08-18
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Mycobacterial reference strains used in this study. [PDF](18 KB)

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Comparison of trees based on the gene and 16S rRNA gene sequences from the same 41 reference strains. [PDF](84 KB)

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Scheme for differentiation of mycobacteria species by PRA of using I and differentiation of mycobacteria strains into five groups by I PRA of DNA. [PDF](50 KB)

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