1887

Abstract

A previously undescribed anaerobic, heterofermentative, non-spore-forming, Gram-positive rod was isolated from the hindgut of the Formosan subterranean termite Shiraki. The DNA G+C content of this bacterium was 37·8 mol%. Sequence analysis of the 16S rRNA gene revealed that this organism is related to, but distinct from, several genera of lactic acid bacteria, principally several species of the genus . Phenotypic traits that serve to separate this organism from related genera include high levels of the cellular fatty acid C18 : 19 and the production of ethanol along with lactic acid as fermentation products. Based on the collected phylogenetic and phenotypic evidence, it is proposed that the unknown organism represents a novel species in a new genus, gen. nov., sp. nov. The type strain is TI-1 (=ATCC BAA-1030=CCUG 49613).

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2006-01-01
2024-03-29
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References

  1. Bauer, S., Tholen, A., Overmann, J. & Brune, A.(2000). Characterization of abundance and diversity of lactic acid bacteria in the hindgut of wood- and soil-feeding termites by molecular and culture-dependent techniques. Arch Microbiol 173, 126–137.[CrossRef] [Google Scholar]
  2. Bosley, G. S., Wallace, P. L., Moss, W., Steigerwalt, A. G., Brenner, D. J., Swenson, J. M., Hebert, G. A. & Facklam, R. R.(1990). Phenotypic characterization, cellular fatty acid composition, and DNA relatedness of aerococci and comparison to related genera. J Clin Microbiol 28, 416–421. [Google Scholar]
  3. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef] [Google Scholar]
  4. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E.(1994). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef] [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  6. Devriese, L. A., Pot, B. & Collins, M. D.(1993). Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J Appl Bacteriol 75, 399–408.[CrossRef] [Google Scholar]
  7. Drancourt, M., Bollet, C., Carlioz, A., Martelin, R., Gayral, J.-P. & Raoult, D.(2000). 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol 38, 3623–3630. [Google Scholar]
  8. Eutick, M. L., O'Brien, R. W. & Slaytor, M.(1978). Bacteria from the gut of Australian termites. Appl Environ Microbiol 35, 823–828. [Google Scholar]
  9. Felsenstein, J.(1989).phylip – Phylogenetic Inference Package, version 3.5. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Hall, T. A.(1999).bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  11. Hugenholtz, P., Pitulle, C., Hershberger, K. L. & Pace, N. R.(1998). Novel division level bacterial diversity in a Yellowstone hot spring. J Bacteriol 180, 366–376. [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  13. Hwang, M. & Ederer, G. M.(1975). Rapid hippurate hydrolysis method for the presumptive identification of group B streptococci. J Clin Microbiol 1, 114–115. [Google Scholar]
  14. Kane, M. D. & Breznak, J. A.(1991).Acetonema longum gen. nov. sp. nov., an H2/CO2 acetogenic bacterium from the termite, Pterotermes occidentus. Arch Microbiol 156, 91–98.[CrossRef] [Google Scholar]
  15. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. New York: Wiley.
  16. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 38, 159–167. [Google Scholar]
  17. Ohkuma, M. & Kudo, T.(1996). Phylogenetic diversity of the intestinal bacterial community in the termite Reticulitermes speratus. Appl Environ Microbiol 62, 461–468. [Google Scholar]
  18. Sasser, M.(1997).Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI.
  19. Schultz, J. E. & Breznak, J. A.(1978). Heterotrophic bacteria present in the hindguts of wood-eating termites [Reticulitermes flavipes (Kollar)]. Appl Environ Microbiol 35, 930–936. [Google Scholar]
  20. Schultz, J. E. & Breznak, J. A.(1979). Cross-feeding of lactate between Streptococcus lactis and Bacteroides sp. isolated from termite hindguts. Appl Environ Microbiol 37, 1206–1210. [Google Scholar]
  21. Stiles, M. E. & Holzapfel, W. H.(1997). Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 36, 1–29.[CrossRef] [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  23. Trinkerl, M., Breunig, A., Schauder, R. & Konig, H.(1990).Desulfovibrio termitidis sp. nov., a carbohydrate-degrading sulfate-reducing bacterium from the hindgut of a termite. Syst Appl Microbiol 13, 372–377.[CrossRef] [Google Scholar]
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