1887

Abstract

A Gram-negative, rod-shaped, motile, non-pigmented, facultative aerobe that grew optimally at pH 6.5 and 30 °C (strain PM1) was isolated for its ability to completely degrade the gasoline additive methyl -butyl ether. Analysis of the 16S rRNA gene sequence indicated that this bacterium was a member of the class in the group. The 16S rRNA gene sequence identity to other genera in this group, , , , , and , ranged from 93 to 96 %. The chemotaxonomic data including Q-8 as the major quinone, C16 : 17 and C16 : 0 as the major fatty acids and a DNA G+C content of 69 mol%, support the inclusion of strain PM1 in the class . It differed from other members of the group by being a facultative methylotroph that used methanol as a sole carbon source, and by also being able to grow heterotrophically in defined media containing ethanol, toluene, benzene, ethylbenzene and dihydroxybenzoates as sole carbon sources. On the basis of the morphological, physiological, biochemical and genetic information, a new genus and species, gen. nov., sp. nov., is proposed, with PM1 (=ATCC BAA-1232=LMG 22953) as the type strain.

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2006-05-01
2020-01-29
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