1887

Abstract

The taxonomic position of an actinomycete strain isolated from soil from Natore, Bangladesh, was examined by using a polyphasic approach. The strain, designated AAB-4, was assigned to the genus on the basis of chemical and morphological criteria. It formed aerial hyphae that carried long chains of rounded spores. The 16S rRNA gene of strain AAB-4 was sequenced directly and then compared with those of previously studied streptomycetes following the generation of two phylogenetic trees by using maximum-likelihood and neighbour-joining algorithms. This confirmed the assignment of the novel strain to the genus . This strain showed a high level of 16S rRNA gene sequence similarity to , and , among others, but could be distinguished from them by phenotypic and physiological traits. This micro-organism produces bis-(2-ethylhexyl)phthalate, an antibacterial and antifungal agent. It is proposed that strain AAB-4 be classified as a novel species within the genus , as sp. nov. (type strain, AAB-4=LMG 22738=NRRL B-24326).

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2005-09-01
2019-12-09
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References

  1. Al-Tai, A., Kim, B., Kim, S. B., Manfio, G. P. & Goodfellow, M. ( 1999; ). Streptomyces malaysiensis sp. nov., a new streptomycete species with rugose, ornamented spores. Int J Syst Bacteriol 49, 1395–1402.[CrossRef]
    [Google Scholar]
  2. Anderson, A. S. & Wellington, E. M. H. ( 2001; ). The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51, 797–814.[CrossRef]
    [Google Scholar]
  3. Barry, A. L. ( 1980; ). Procedure for testing antimicrobial agents in agar media: theoretical consideration. In Antibiotics in Laboratory Medicine, pp. 1–23. Edited by V. Lorian. Baltimore: Williams & Wilkins.
  4. Bérdy, J. ( 1995; ). Are actinomycetes exhausted as a source of secondary metabolites? Biotechnologia 7–8, 13–34.
    [Google Scholar]
  5. Beur, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M. ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45, 493–496.
    [Google Scholar]
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Hopwood, D. A. ( 1967; ). Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev 31, 373–403.
    [Google Scholar]
  8. Hunter, I. S. ( 1988; ). Gene cloning in Streptomyces. In DNA Cloning: a Practical Approach, vol. 2, pp. 19–44. Edited by D. M. Glover. Washington, DC: IRL Press.
  9. Kim, S.-K., Kim, S.-S. & Kim, C.-H. ( 1990; ). New antibiotics produced by Streptomyces melanosporofaciens. I. Taxonomy of the producing microorganism. Kor J Appl Microbiol Biotechnol 18, 624–632.
    [Google Scholar]
  10. Kim, S.-K., Kim, S.-S., Kim, K.-S., Chung, Y.-R. & Kim, C.-H. ( 1991; ). New antibiotics produced by Streptomyces melanosporofaciens. II. Antimicrobial activities and isolation, purification, and structure determination of the active compound. Kor J Appl Microbiol Biotechnol 19, 235–241.
    [Google Scholar]
  11. Kim, B., Sahin, N., Minnikin, D. E., Zakrzewska-Czerwinska, J., Mordarski, M. & Goodfellow, M. ( 1999; ). Classification of thermophilic streptomycetes, including the description of Streptomyces thermoalcalitolerans sp. nov. Int J Syst Bacteriol 49, 7–17.[CrossRef]
    [Google Scholar]
  12. Kim, B., Al-Tai, A. M., Kim, S. B., Somasundaram, P. & Goodfellow, M. ( 2000; ). Streptomyces thermocoprophilus sp. nov., a cellulase-free endo-xylanase-producing streptomycete. Int J Syst Evol Microbiol 50, 505–509.[CrossRef]
    [Google Scholar]
  13. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  14. Korn-Wendish, F. & Kutzner, H. J. ( 1992; ). The family Streptomycetaceae. In The Prokaryotes, pp. 921–995. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  15. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetic analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  16. Omilian, A. R. & Taylor, D. J. ( 2001; ). Rate acceleration and long-branch attraction in a conserved gene of cryptic daphniid (Crustacea) species. Mol Biol Evol 18, 2201–2212.[CrossRef]
    [Google Scholar]
  17. Petrosyan, P., García-Varela, M., Luz-Madrigal, A., Huitrón, C. & Flores, M. E. ( 2003; ). Streptomyces mexicanus sp. nov., a xylanolytic micro-organism isolated from soil. Int J Syst Evol Microbiol 53, 269–273.[CrossRef]
    [Google Scholar]
  18. Posada, D. & Crandall, K. A. ( 1998; ). modeltest: testing the model of DNA substitution. Bioinformatics 9, 817–818.
    [Google Scholar]
  19. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  20. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  21. Swofford, D. L. ( 2002; ). paup*: Phylogenetic Analysis Using Parsimony (and other methods), version 4.0b10. Sunderland, MA: Sinauer Associates.
  22. Tamura, K. & Nei, M. ( 1993; ). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10, 512–526.
    [Google Scholar]
  23. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  24. Waksman, S. A. & Henrici, A. T. ( 1943; ). The nomenclature and classification of the actinomycetes. J Bacteriol 46, 337–341.
    [Google Scholar]
  25. Williams, S. T., Goodfellow, M. & Alderson, G. ( 1989; ). Genus Streptomyces Waskman and Henrici 1943 , 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
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vol. , part 5, pp. 1973 – 1977

Neighbour-joining tree recovered from analysis of 16S rRNA gene sequence dataset. Numbers near internal nodes show the bootstrap values based on 10 000 replicates [PDF](662 KB)



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