The taxonomic position of the free-living diazotrophic strain COC8T isolated from rice was investigated based on phylogenetic analyses. 16S rRNA gene sequence analyses indicated that strain COC8T was closely related to the genus Azospirillum (96 % similarity). Chemotaxonomic characteristics (G+C content of the DNA 66·8 mol%, Q-10 quinone system, 18 : 1ω7c as the major fatty acid and 14 : 0 3-OH and 16 : 0 3-OH as the major hydroxy fatty acids) were also similar to those of the genus Azospirillum. Based on its physiological characteristics, strain COC8T can clearly be differentiated from recognized species of Azospirillum. The name Azospirillum oryzae sp. nov. is proposed to accommodate this bacterial strain; the type strain is COC8T (=IAM 15130T=CCTCC AB204051T).
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