1887

Abstract

The taxonomic position of the free-living diazotrophic strain COC8 isolated from rice was investigated based on phylogenetic analyses. 16S rRNA gene sequence analyses indicated that strain COC8 was closely related to the genus (96 % similarity). Chemotaxonomic characteristics (G+C content of the DNA 66·8 mol%, Q-10 quinone system, 18 : 17 as the major fatty acid and 14 : 0 3-OH and 16 : 0 3-OH as the major hydroxy fatty acids) were also similar to those of the genus . Based on its physiological characteristics, strain COC8 can clearly be differentiated from recognized species of . The name sp. nov. is proposed to accommodate this bacterial strain; the type strain is COC8 (=IAM 15130=CCTCC AB204051).

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2005-07-01
2020-01-25
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References

  1. Ben Dekhil, S., Cahill, M., Stackebrandt, E. & Sly, L. I. ( 1997; ). Transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum as Azospirillum largimobile comb. nov., and elevation of Conglomeromonas largomobilis subsp. parooensis to the new type species of Conglomeromonas, Conglomeromonas parooensis sp. nov. Syst Appl Microbiol 20, 72–77.[CrossRef]
    [Google Scholar]
  2. Eckert, B., Weber, O. B., Kirchhof, G., Halbritter, A., Stoffels, M. & Hartmann, A. ( 2001; ). Azospirillum doebereinerae sp. nov., a nitrogen-fixing bacterium associated with the C4-grass Miscanthus. Int J Syst Evol Microbiol 51, 17–26.
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Khammas, K. M., Ageron, E., Grimont, P. A. D. & Kaiser, P. ( 1989; ). Azospirillum irakense sp. nov., a nitrogen-fixing bacterium associated with rice roots and rhizosphere soil. Res Microbiol 140, 679–693.
    [Google Scholar]
  5. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  6. Ladha, J. K., So, R. B. & Watanabe, I. ( 1987; ). Composition of Azospirillum species associated with wetland rice plants grown in different soils. Plant Soil 102, 127–129.[CrossRef]
    [Google Scholar]
  7. Oyaizu-Masuchi, Y. & Komagata, K. ( 1988; ). Isolation of free-living nitrogen-fixing bacteria from the rhizosphere of rice. J Gen Appl Microbiol 34, 127–164.[CrossRef]
    [Google Scholar]
  8. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  9. Skerman, V. B. D., Sly, L. I. & Williamson, M. ( 1983; ). Conglomeromonas largomobilis gen. nov., sp. nov., a sodium-sensitive, mixed-flagellated organism from freshwater. Int J Syst Bacteriol 33, 300–308.[CrossRef]
    [Google Scholar]
  10. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  11. Swofford, D. L. ( 1998; ). paup* (Phylogenetic Analysis Using Parsimony* and other methods), version 4. Sunderland, MA: Sinauer Associates.
  12. Tarrand, J. J., Krieg, N. R. & Dobereiner, J. ( 1978; ). A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov., and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol 24, 967–980.[CrossRef]
    [Google Scholar]
  13. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  14. Xie, C.-H. & Yokota, A. ( 2003; ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef]
    [Google Scholar]
  15. Xie, C.-H. & Yokota, A. ( 2004; ). Phylogenetic analyses of the nitrogen-fixing genus Derxia. J Gen Appl Microbiol 50, 129–135.[CrossRef]
    [Google Scholar]
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