1887

Abstract

Three strains of Gram-negative, aerobic, yellow-pigmented, chemo-organotrophic bacteria, motile by a polar flagellum, were isolated from the rhizosphere of spring barley ( L.) at a research field near Copenhagen, Denmark. The three strains, LJ79, LJ96 and LJ99, formed visible colonies on one-tenth-strength tryptic soy broth supplemented with agar (1/10 TSBA) after incubation for 6 days at 15 °C. The strains hydrolysed starch, casein (skimmed milk), gelatin and various pentoses and hexoses and grew on MacConkey agar and full-strength TSBA. Growth on 1/10 TSBA occurred at 4–30 °C, pH 6–9 and 0–3 % (w/v) NaCl. The strains had identical 16S rRNA gene sequences and ERIC (enterobacterial repetitive intergenic consensus sequence) fingerprint profiles, but could be differentiated by their RAPD (random amplified polymorphic DNA) fingerprint patterns. Strain LJ96 had a DNA G+C content of 64·3 mol% and the major fatty acids were 15 : 0 iso (23·4 %), 17 : 1 iso 9 (25·5 %) and 17 : 0 iso (18·1 %). Phylogenetic analysis of the 16S rRNA gene sequences of the three strains showed 96 % sequence similarity to s LMG 18385, 95 % to DSM 6220 and 96 % to LMG 19981. Using LJ96 DNA as probe, DNA–DNA hybridizations documented the relationship of the three strains to a single species (87·4–98·7 % relatedness) and showed less than 30 % relatedness to DSM 6220 and DSM 14263. s LMG 18385 is not extant and the strain not available from any public strain collections, thus DNA–DNA hybridization could not include this strain. On the basis of genotypic and phenotypic characteristics, the three yellow-pigmented strains could also be differentiated from , and s. The name gen. nov., sp. nov. is proposed, with the type strain LJ96 (=DSM 16549=ATCC BAA-1015).

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2005-11-01
2019-09-19
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Cellular fatty acid composition (%) of LJ96 and DSM 14263 . [PDF](20 KB)

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ERIC-PCR (a) and RAPD (b) fingerprints of gen. nov. and relatives. Lanes: L, 0.1 kbp ladder; 1, DSM 14263 ; 2, DSM 6220 ; 3, LJ99; 4, LJ96 ; 5, LJ79.

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