1887

Abstract

During the course of a 16S rRNA gene sequence phylogenetic evaluation of putative strains, it was noted that strain NRRL B-16338 is phylogenetically nearest to the genus but apparently is not a member of this or any of the other currently described actinomycete genera. The strain was subjected to a polyphasic study using standard methods for chemotaxonomic, morphological and physiological evaluation. The strain exhibited chemotaxonomic characteristics distinct from in spite of having 16S rRNA gene sequence similarity of 92 % with the described species of this genus. The whole-cell sugar pattern of NRRL B-16338 consisted of ribose and inositol, with traces of arabinose and mannose. The phospholipids observed were phosphatidylglycerol and diphosphatidylglycerol and menaquinones consisting of MK-10(H), MK-10(H), MK-11(H) and MK-11(H). A significant quantity (14·5 %) of 17 : 0 anteiso 2-hydroxy fatty acid was observed in the fatty acid profile of this strain. These characteristics clearly differentiate NRRL B-16338 from members of the genus and it is proposed that the strain represents a new genus within the family to be called gen. nov. The description of this family is emended to permit its inclusion. It is proposed that the type species of the genus should be named . The type strain LLR-40K-21 (=NRRL B-16338=DSM 44728) was isolated from a soil sample from Nassau, Bahamas.

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2005-07-01
2024-03-28
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References

  1. DSMZ 2001 Catalogue of Strains Braunschweig: Deutsche Sammlung von Mikroorganismen und Zellkulturen;
    [Google Scholar]
  2. Evtushenko L. I., Taptykova S. D., Akimov V. N., Semyonova S. A., Kalakoutskii L. V. 1991; Glycomyces tenuis sp. nov. Int J Syst Bacteriol 41:154–157 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1989 phylip (Phylogeny Inference Package), version 3.5.1. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, WA, USA:
    [Google Scholar]
  4. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. -N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  5. Grund E., Kroppenstedt R. M. 1989; Transfer of five Nocardiopsis species to the genus Saccharothrix Labeda et al ., 1984. Syst Appl Microbiol 12:267–274 [CrossRef]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  7. Kurup P. V., Schmitt J. A. 1973; Numerical taxonomy of Nocardia . Can J Microbiol 19:1035–1048 [CrossRef]
    [Google Scholar]
  8. Labeda D. P., Kroppenstedt R. M. 2000; Phylogenetic analysis of Saccharothrix and related taxa: proposal for Actinosynnemataceae fam. nov. Int J Syst Evol Microbiol 50:331–336 [CrossRef]
    [Google Scholar]
  9. Labeda D. P., Kroppenstedt R. M. 2004; Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp.nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol 54:2343–2346 [CrossRef]
    [Google Scholar]
  10. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A. 1985; Glycomyces , a new genus of the Actinomycetales . Int J Syst Evol Microbiol 35:417–421
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson A., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  13. Pridham T. G., Lyons A. J. 1980; Methodologies for Actinomycetales with special reference to streptomycetes and streptoverticillia. In Actinomycete Taxonomy pp  153–224 Edited by Dietz A., W D. Thayer. Special publication no 6 Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  14. Saddler G. S., Tavecchia P., Lociuro S., Zanol M., Colombo E., Selva E. 1991; Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14:185–191 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6
    [Google Scholar]
  17. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  18. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  19. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  20. Uchida K., Aida K. 1977; Acyl type of bacterial cell walls: its simple identification by colorimetric methods. J Gen Appl Microbiol 23:249–260 [CrossRef]
    [Google Scholar]
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