1887

Abstract

A Gram-negative, motile, slightly halophilic bacterial strain, SW-150, was isolated from sea water of the Yellow Sea, Korea, and was characterized by a polyphasic taxonomic approach. Strain SW-150 grew optimally at 25–30 °C and in the presence of 2 % (w/v) NaCl. The isolate could be distinguished from other species in producing an orange pigment. It contained ubiquinone-10 as the predominant respiratory lipoquinone and C 7 and C 6 as the major fatty acids. No 3-hydroxy fatty acids were detected. Major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 63 mol%. Comparative 16S rRNA gene sequence analyses showed that strain SW-150 was phylogenetically affiliated to the genus of the family . Similarity values between the 16S rRNA gene sequences of strain SW-150 and the type strains of species ranged from 91·6 to 94·2 %, making it possible to categorize strain SW-150 as a species that is separate from previously described species. On the basis of phenotypic properties and phylogenetic distinctiveness, SW-150 (=KCTC 12231=DSM 16222) should be classified as the type strain of a novel species, for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63495-0
2005-05-01
2020-09-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/3/ijs551223.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63495-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium ,Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes . pp  1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  3. Busse H.-J., Denner E. B. M., Buczolits S., Salkinoja-Salonen M., Bennasar A., Kämpfer P. 2003; Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov.,air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas . Int J Syst Evol Microbiol 53:1253–1260 [CrossRef]
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Fujii K., Satomi M., Morita N., Motomura T., Tanaka T., Kikuchi S. 2003; Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. Int J Syst Evol Microbiol 53:47–52 [CrossRef]
    [Google Scholar]
  7. Godoy F., Vancanneyt M., Martínez M., Steinbüchel A., Swings J., Rehm B. H. A. 2003; Sphingopyxis chilensis sp. nov., a chlorophenol-degrading bacterium that accumulates polyhydroxyalkanoate, and transfer of Sphingomonas alaskensis to Sphingopyxis alaskensis comb. nov. Int J Syst Evol Microbiol 53:473–477 [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Denner E. B. M., Meyer S., Moore E. R. B., Busse H.-J. 1997; Classification of “ Pseudomonas azotocolligans ” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47:577–583 [CrossRef]
    [Google Scholar]
  9. Kämpfer P., Witzenberger R., Denner E. B. M., Busse H.-J., Neef A. 2002; Sphingopyxis witflariensis sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 52:2029–2034 [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K.-I. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  11. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Lee J.-S., Shin Y. K., Yoon J.-H., Takeuchi M., Pyun Y.-R., Park Y.-H. 2001; Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov. and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Evol Microbiol 51:1491–1498
    [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  14. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  15. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI;
    [Google Scholar]
  16. Sohn J. H., Kwon K. K., Kang J.-H., Jung H.-B., Kim S.-J. 2004; Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54:1483–1487 [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  18. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  19. Takeuchi M., Kawai F., Shimada Y., Yokota A. 1993; Taxonomic study of polyethylene glycol-utilizing bacteria: emended description of the genus Sphingomonas and new descriptions of Sphingomonas macrogoltabidus sp. nov., Sphingomonas sanguis sp. nov. and Sphingomonas terrae sp. nov.. Syst Appl Microbiol 16:227–238 [CrossRef]
    [Google Scholar]
  20. Takeuchi M., Sakane T., Yanagi M., Yamasato K., Hamana K., Yokota A. 1995; Taxonomic study of bacteria isolated from plants: proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp. nov., and Sphingomonas mali sp. nov.. Int J Syst Bacteriol 45:334–341 [CrossRef]
    [Google Scholar]
  21. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera,Sphingobium , Novosphingobium and Sphingopyxis , on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417
    [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  23. Ushiba Y., Takahara Y., Ohta H. 2003; Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment. Int J Syst Evol Microbiol 53:2045–2048 [CrossRef]
    [Google Scholar]
  24. Vancanneyt M., Schut F., Snauwaert C., Goris J., Swings J., Gottschal J. C. 2001; Sphingomonas alaskensis sp. nov., a dominant bacterium from a marine oligotrophic environment. Int J Syst Evol Microbiol 51:73–79
    [Google Scholar]
  25. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. 1990; Proposals of Sphingomonas paucimobilis gen.nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov.,Sphingomonas adhaesiva sp. nov., Sphingomonascapsulate comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34:99–119 [CrossRef]
    [Google Scholar]
  26. Yabuuchi E., Kosako Y., Fujiwara N., Naka T., Matsunaga I., Ogura H., Kobayashi K. 2002; Emendation of the genus Sphingomonas Yabuuchi et al . 1990 and junior objective synonymy of the species of three genera, Sphingobium , Novosphingobium and Sphingopyxis , in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 52:1485–1496 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Oh T.-K. 2005; Sphingopyxis flavimaris sp. nov., isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55:369–373 [CrossRef]
    [Google Scholar]
  28. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  29. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  30. Yoon J.-H., Kim H., Kim I.-G., Kang K. H., Park Y.-H. 2003; Erythrobacter flavus sp. nov., a slight halophile from the East Sea in Korea. Int J Syst Evol Microbiol 53:1169–1174 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63495-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63495-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error