1887

Abstract

A novel alkaliphilic, sulfate-reducing bacterium was isolated from a syntrophic acetate-decomposing community enriched from samples of the soda lake Khadin, Tuva, Russia; the isolate was designated strain Z-7999. Cells of strain Z-7999 were vibrioid, Gram-negative, 0·4–0·5×1·0–2·5 μm and motile by means of a polar flagellum. The temperature range for growth was 15–40 °C, with an optimum of 35–38 °C. The pH range for growth was 6·7–10·3, with an optimum of pH 8·0–9·0. The NaCl concentration range for growth was 1–80 g l. The novel isolate was obligately anaerobic, was alkaliphilic with a broad pH range and had an obligate requirement for carbonate ions in the growth medium. In the presence of sulfate as electron acceptor, it grew with hydrogen, formate and lactate. It was not able to ferment sugars, organic acids, amino acids or peptides. During growth on formate, strain Z-7999 reduced sulfite and thiosulfate to sulfide. It was able to grow lithoheterotrophically with sulfate and formate when acetate was added as a carbon source for biosynthesis of biomass. The G+C content of the genomic DNA of strain Z-7999 was 56·5 mol%. Results of comparative 16S rRNA gene sequence analyses revealed that strain Z-7999 was part of the - and clustered with other members of the genus (similarity values of 95·2 and 95·3 % to and , respectively). DNA–DNA hybridization with was 37 %. On the basis of physiological and phylogenetic data, it is proposed that strain Z-7999 (=DSM 16749=VKM B-2329) should be placed in the genus as a representative of a novel species, sp. nov.

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2005-05-01
2020-01-23
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