1887

Abstract

A moderately halophilic, Gram-positive, rod-shaped bacterium (BH030004) was isolated from a solar saltern in Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BH030004 belonged to the genus . Chemotaxonomic data (DNA G+C content, 42 mol%; major isoprenoid quinone, MK-7; cell-wall type, A1-type -diaminopimelic acid; major fatty acids, iso-C and anteiso-C) also supported the affiliation of the isolate to the genus . Although the 16S rRNA gene sequence similarity between strain BH030004 and DSM 16287 was relatively high (99·1 %), physiological properties and DNA–DNA hybridization (about 7 % DNA–DNA relatedness) allowed genotypic and phenotypic differentiation of strain BH030004 from the type strain of . Therefore, strain BH030004 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BH030004 (=KCTC 3917=DSM 16465).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63489-0
2005-05-01
2024-10-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/3/ijs551027.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63489-0&mimeType=html&fmt=ahah

References

  1. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit ribosomal-RNA sequences. Lett Appl Microbiol 13:202–206
    [Google Scholar]
  2. Cole J. R., Chai B., Marsh T. L. 8 other authors 2003; The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
    [Google Scholar]
  3. DeLong E. F. 1992; Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89:5685–5689 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a Seattle: University of Washington;
    [Google Scholar]
  5. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A. 1998; Virgibacillus : a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus . Int J Syst Bacteriol 48:99–106 [CrossRef]
    [Google Scholar]
  6. Heyrman J., Logan N. A., Busse H. J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P. 2003; Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus , as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus . Int J Syst Evol Microbiol 53:501–511 [CrossRef]
    [Google Scholar]
  7. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–208
    [Google Scholar]
  8. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  9. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  10. Lim J.-M., Jeon C. O., Song S. M., Kim C.-J. 2005; Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55:165–170 [CrossRef]
    [Google Scholar]
  11. Nielsen P., Rainey F. A., Outtrup H., Priest F. G., Fritze D. 1994; Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus . FEMS Microbiol Lett 117:61–66 [CrossRef]
    [Google Scholar]
  12. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Völker H., Thomm M. 2001; Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn–d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51:425–431
    [Google Scholar]
  13. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K. H. 1996; Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
    [Google Scholar]
  14. Stackebrandt E., Liesack W. 1993; Nucleic acids and classification. In Handbook of New Bacterial Systematics pp  152–189 Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;
    [Google Scholar]
  15. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  16. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. 1999; Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49:821–831 [CrossRef]
    [Google Scholar]
  19. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  20. Yoon J. H., Kang K. H., Park Y. H. 2002; Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 52:2043–2048 [CrossRef]
    [Google Scholar]
  21. Yoon J. H., Kang K. H., Park Y. H. 2003; Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53:687–693 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63489-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63489-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error