1887

Abstract

A halophilic and halotolerant, facultatively alkaliphilic strain, K11, was isolated from soil obtained from Oshyamanbe, Oshima, Hokkaido, Japan. The isolate grew at pH 7–10. It was non-motile, Gram-positive and aerobic. Cells comprised straight rods and produced ellipsoidal spores. The isolate grew in 0–20 % NaCl, with optimum growth at 7 % NaCl, and hydrolysed casein, gelatin, starch, DNA and Tweens 20, 40, 60 and 80. The major isoprenoid quinone was menaquinone-7, and the cellular fatty acid profile consisted of significant amounts of C branched-chain acids, iso C and anteiso C. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain K11 was a member of group 6 [ Nielsen ., (1994), 61–66 ] (alkaliphiles) of the genus . DNA–DNA hybridization revealed a low relatedness (14 %) of the isolate to its closest phylogenetic neighbour, . On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic data and DNA–DNA relatedness data, it was concluded that K11 (=JCM 12663=NCIMB 14023) merits classification as the type strain of a novel species, for which the name sp. nov. is proposed.

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2005-03-01
2021-03-05
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