RT Journal Article SR Electronic(1) A1 Nakajima, Kenji A1 Hirota, Kikue A1 Nodasaka, Yoshinobu A1 Yumoto, IsaoYR 2005 T1 Alkalibacterium iburiense sp. nov., an obligate alkaliphile that reduces an indigo dye JF International Journal of Systematic and Evolutionary Microbiology, VO 55 IS 4 SP 1525 OP 1530 DO https://doi.org/10.1099/ijs.0.63487-0 PB Microbiology Society, SN 1466-5034, AB Three indigo-reducing obligately alkaliphilic strains, M3T, 41A and 41C, were isolated. The isolates grew at pH 9–12, but not at pH 7–8. They were Gram-positive, facultatively anaerobic, straight rod-shaped strains with peritrichous flagella. The isolates grew in 0–14 % (w/v) NaCl, with optimum growth at 3–13 %. They grew at temperatures between 10 and 45 °C, with optimum growth at around 30–37 °C. They did not hydrolyse starch or gelatin. dl-lactate was the major end-product from d-glucose. No quinones could be detected. The peptidoglycan type was A4β, Orn–d-Asp. The major cellular fatty acids were C16 : 0, C16 : 17c and C18 : 19c. The DNA G+C content was 42·6–43·2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the isolates belong to the genus Alkalibacterium. DNA–DNA hybridization revealed low similarity (less than 16 %) of the isolates with respect to the two closest phylogenetically related strains, Alkalibacterium olivapovliticus and Alkalibacterium psychrotolerans. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic data and DNA–DNA relatedness, the isolates merit classification as a novel species of the genus Alkalibacterium, for which the name Alkalibacterium iburiense is proposed. The type strain is M3T (=JCM 12662T=NCIMB 14024T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63487-0