1887

Abstract

Three indigo-reducing obligately alkaliphilic strains, M3, 41A and 41C, were isolated. The isolates grew at pH 9–12, but not at pH 7–8. They were Gram-positive, facultatively anaerobic, straight rod-shaped strains with peritrichous flagella. The isolates grew in 0–14 % (w/v) NaCl, with optimum growth at 3–13 %. They grew at temperatures between 10 and 45 °C, with optimum growth at around 30–37 °C. They did not hydrolyse starch or gelatin. -lactate was the major end-product from -glucose. No quinones could be detected. The peptidoglycan type was A4, Orn–-Asp. The major cellular fatty acids were C, C7 and C9. The DNA G+C content was 42·6–43·2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the isolates belong to the genus . DNA–DNA hybridization revealed low similarity (less than 16 %) of the isolates with respect to the two closest phylogenetically related strains, and . On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic data and DNA–DNA relatedness, the isolates merit classification as a novel species of the genus , for which the name is proposed. The type strain is M3 (=JCM 12662=NCIMB 14024).

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2005-07-01
2021-03-04
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