1887

Abstract

A halotolerant, obligately alkaliphilic bacterium, R-2, was isolated from the skin of a rainbow trout (), a freshwater fish. The strain is Gram-positive, ferments several carbohydrates, is rod-shaped and motile by peritrichous flagella and produces ellipsoidal spores. The isolate grows at pH 9–10 but not at pH 7–8. This micro-organism grows in 0–22 % (w/v) NaCl at pH 10. Its major cellular fatty acids are iso-C, anteiso-C and anteiso-C, the major isoprenoid quinone is MK-7 and the DNA G+C content is 38·5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain R-2 is a member of the genus . DNA–DNA hybridization reveals low relatedness between the isolate and (21·0 %). On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, the isolate should be designated as a novel species, for which the name sp. nov. is proposed. The type strain is R-2 (=JCM 12661=NCIMB 14022).

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2005-07-01
2024-04-19
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References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Duckworth A. W., Grant W. D., Jones B. E., Steenbergen R. 1996; Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Lett 19:181–191 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A. 1998; Virgibacillus : a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus . Int J Syst Bacteriol 48:99–106 [CrossRef]
    [Google Scholar]
  5. Horikoshi K. 1991 Microorganisms in Alkaline Environments Weinheim: VCH;
    [Google Scholar]
  6. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Krulwich T. A., Guffanti A. A. 1989; Alkalophilic bacteria. Annu Rev Microbiol 43:435–463 [CrossRef]
    [Google Scholar]
  9. Krulwich T. A., Ito M., Guffanti A. A. 2001; The Na+-dependence of alkaliphily in Bacillus . Biochim Biophys Acta 1505158–168 [CrossRef]
    [Google Scholar]
  10. Lu J., Nogi Y., Takami H. 2001; Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205:291–297 [CrossRef]
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  12. Nazina T. N., Tourova T. P., Poltaraus A. B. 8 other authors 2001; Taxonomic study of aerobic thermophilic bacilli: description of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus , Bacillus thermocatenulatus , Bacillus thermoleovorans , Bacillus kaustophilus , Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus , G. thermocatenulatus , G. thermoleovorans , G. kaustophilus , G.thermoglucosidasius and G. thermodenitrificans . Int J Syst Evol Microbiol 51:433–446
    [Google Scholar]
  13. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [CrossRef]
    [Google Scholar]
  14. Pikuta E., Lysenko A., Chuvilskaya N., Mendrock U., Hippe H., Suzina N., Nikitin D., Osipov G., Laurinavichius K. 2000; Anoxybacillus pushchinensis gen. nov., sp. nov. a novel anaerobic alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov. Int J Syst Evol Microbiol 50:2109–2117 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Spring S., Ludwig W., Márquez M. C., Ventose A., Schleifer K. H. 1996; Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp.nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
    [Google Scholar]
  17. Takami H., Inoue A., Fujii F., Horikoshi K. 1997; Microbial flora in the deepest sea mud of the Mariana Trench. FEMS Microbiol Lett 152:279–285 [CrossRef]
    [Google Scholar]
  18. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  19. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  20. Thongaram T., Kosono S., Ohkuma M., Hongoh Y., Kitada M., Yoshinaka T., Trakulnaleamsai S., Noparatnaraporn N., Kudo T. 2003; Gut of higher termites as a niche for alkaliphiles as shown by culture-based and culture-independent studies. Microbes Environ 18:152–159 [CrossRef]
    [Google Scholar]
  21. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. 1999; Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49:821–831 [CrossRef]
    [Google Scholar]
  22. Yumoto I. 2002; Bioenergetics of alkaliphilic Bacillus spp. J Biosci Bioeng 93:342–353 [CrossRef]
    [Google Scholar]
  23. Yumoto I. 2003; Electron transport system in alkaliphilic Bacillus spp. Recent Res Devel Bacteriol 1:131–149
    [Google Scholar]
  24. Yumoto I., Yamazaki K., Sawabe T., Nakano K., Kawasaki K., Ezura Y., Shinano H. 1998; Bacillus horti sp. nov., a new Gram-negative alkaliphilic bacillus. Int J Syst Bacteriol 48:565–571 [CrossRef]
    [Google Scholar]
  25. Yumoto I., Yamazaki K., Hishinuma M., Nodasaka Y., Suemori A., Nakajima K., Inoue N., Kawasaki K. 2001; Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. Int J Syst Evol Microbiol 51:349–355
    [Google Scholar]
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