1887

Abstract

A halotolerant, obligately alkaliphilic bacterium, R-2, was isolated from the skin of a rainbow trout (), a freshwater fish. The strain is Gram-positive, ferments several carbohydrates, is rod-shaped and motile by peritrichous flagella and produces ellipsoidal spores. The isolate grows at pH 9–10 but not at pH 7–8. This micro-organism grows in 0–22 % (w/v) NaCl at pH 10. Its major cellular fatty acids are iso-C, anteiso-C and anteiso-C, the major isoprenoid quinone is MK-7 and the DNA G+C content is 38·5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain R-2 is a member of the genus . DNA–DNA hybridization reveals low relatedness between the isolate and (21·0 %). On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, the isolate should be designated as a novel species, for which the name sp. nov. is proposed. The type strain is R-2 (=JCM 12661=NCIMB 14022).

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2005-07-01
2020-01-23
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