1887

Abstract

A novel solvent-producing, anaerobic clostridium, strain P7, was isolated from sediment from an agricultural settling lagoon after enrichment with CO as the substrate. The metabolism of this Gram-positive, motile, spore-forming rod was primarily acetogenic. Acetate, ethanol, butyrate and butanol were the end-products of metabolism. Strain P7 grew on CO, H/CO, glucose, galactose, fructose, xylose, mannose, cellobiose, trehalose, cellulose, starch, pectin, citrate, glycerol, ethanol, propanol, 2-propanol, butanol, glutamate, aspartate, alanine, histidine, asparagine, serine, betaine, choline and syringate as sole substrates. Growth was not supported by methanol, formate, -arabinose, fucose, lactose, melibiose, amygdalin, gluconate, lactate, malate, arginine, glutamine or vanillate. Nitrate reduction, production of indole, gelatin hydrolysis and aesculin hydrolysis were not observed. Analysis of the 16S rRNA gene sequence of the isolate showed that it was closely related to ATCC 25775 (99·7 % sequence similarity) and clostridial strain SL1 (99·8 % sequence similarity). Strain SL1 had been classified as a strain of . However, DNA–DNA reassociation analysis showed that both strain P7 and strain SL1 represented novel clostridial species. It is proposed that strain P7 (=ATCC BAA-624=DSM 15243) be classified as the type strain of sp. nov. and that strain SL1 (=ATCC BAA-623=DSM 12750) be reclassified as the type strain of sp. nov.

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2005-09-01
2019-10-19
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References

  1. Adler, H. I. & Crow, W. ( 1987; ). A technique for predicting the solvent-producing ability of Clostridium acetobutylicum. Appl Environ Microbiol 53, 2496–2499.
    [Google Scholar]
  2. Balch, W. E. & Wolfe, R. S. ( 1976; ). New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32, 781–791.
    [Google Scholar]
  3. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  4. Buschhorn, H., Durre, P. & Gottschalk, G. ( 1989; ). Production and utilization of ethanol by the homoacetogen Acetobacterium woodii. Appl Environ Microbiol 55, 1835–1840.
    [Google Scholar]
  5. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  6. Cato, E. P., George, W. L. & Finegold, S. M. ( 1986; ). Genus Clostridium Prazmowski 1880, 23AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1141–1200. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  7. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  8. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  9. Drake, H. L. (editor) ( 1994; ). Acetogenesis. New York: Chapman & Hall.
  10. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. ( 1956; ). Colorimetric method for determination of sugars and related substances. Anal Chem 28, 350–356.[CrossRef]
    [Google Scholar]
  11. Escara, J. F. & Hutton, J. R. ( 1980; ). Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19, 1315–1327.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  13. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  14. Fox, G. E., Wisotzkey, J. D. & Jurtshuk, P., Jr ( 1992; ). How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int J Syst Bacteriol 42, 166–170.[CrossRef]
    [Google Scholar]
  15. Genthner, B. R. S., Davis, C. L. & Bryant, M. P. ( 1981; ). Features of rumen and sewage sludge strains of Eubacterium limosum, a methanol- and H2–CO2-utilizing species. Appl Environ Microbiol 42, 12–19.
    [Google Scholar]
  16. Grethlein, A. J., Worden, R. M., Jain, M. K. & Datta, R. ( 1991; ). Evidence for production of n-butanol from carbon monoxide by Butyribacterium methylotrophicum. J Ferment Bioeng 72, 58–60.[CrossRef]
    [Google Scholar]
  17. Gutell, R. R., Larsen, N. & Woese, C. R. ( 1994; ). Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective. Microbiol Rev 58, 10–26.
    [Google Scholar]
  18. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute & State University.
  19. Hungate, R. E. ( 1969; ). A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  20. Huß, V. A. R., Festl, H. & Schleifer, K.-H. ( 1983; ). Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  21. Jahnke, K.-D. ( 1992; ). Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  22. Johnson, J. L. & Francis, B. S. ( 1975; ). Taxonomy of the clostridia: ribosomal ribonucleic acid homologies among the species. J Gen Microbiol 88, 229–244.[CrossRef]
    [Google Scholar]
  23. Kusel, K., Dorsch, T., Acker, G., Stackebrandt, E. & Drake, H. L. ( 2000; ). Clostridium scatologenes strain SL1 isolated as an acetogenic bacterium from acidic sediments. Int J Syst Evol Microbiol 50, 537–546.[CrossRef]
    [Google Scholar]
  24. Ludwig, W. ( 1991; ). DNA sequencing in bacterial systematics. In Nucleic Acid Techniques in Bacterial Systematics, pp. 69–95. Edited by E. Stackebrandt & M. Goodfellow. New York: Wiley.
  25. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  26. Olsen, G. J., Matsuda, H., Hagström, R. & Overbeek, R. ( 1994; ). fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10, 41–48.
    [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  28. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  29. Stackebrandt, E. & Hippe, H. ( 2001; ). Taxonomy and systematics. In Clostridia, pp. 19–48. Edited by H. Bahl & P. Dürre. New York: Wiley-VCH.
  30. Swofford, D. L. ( 2000; ). paup 4.0 beta, version 4.0b4a. Sunderland, MA: Sinauer Associates.
  31. Tanner, R. S. ( 2002; ). Cultivation of bacteria and fungi. In Manual of Environmental Microbiology, 2nd edn, pp. 62–70. Edited by C. J. Hurst, R. L. Crawford, G. R. Knudsen, M. J. McInerney & L. D. Stetzenbach. Washington, DC: American Society for Microbiology.
  32. Tanner, R. S., Miller, L. M. & Yang, D. ( 1993; ). Clostridium ljungdahlii sp. nov., an acetogenic species in clostridial rRNA homology group I. Int J Syst Bacteriol 43, 232–236.[CrossRef]
    [Google Scholar]
  33. Vega, J. L., Prieto, S., Elmore, B. B., Clausen, E. C. & Gaddy, J. L. ( 1989; ). The biological production of ethanol from synthesis gas. Appl Biochem Biotechnol 20/21, 781–797.[CrossRef]
    [Google Scholar]
  34. Versalovic, J., Schneider, M., de Bruijn, F. J. & Lupski, J. R. ( 1994; ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40.
    [Google Scholar]
  35. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  36. Weisberg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  37. Worden, R. M., Grethlein, A. J., Jain, M. K. & Datta, R. ( 1991; ). Production of butanol and ethanol from synthesis gas via fermentation. Fuel 70, 615–619.[CrossRef]
    [Google Scholar]
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Transmission electron micrograph of a pair of negatively stained cells of strain P7 . Bar, 1 µm.

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BOX-PCR fingerprints of ATCC 25775 (lane 2), strain SL1 (lane 3) and strain P7 (lane 4). A DNA standard (2072-400 bp) is shown in lane 1 and a gel control is shown in lane 5.

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