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Volume 55, Issue 2

gen. nov., sp. nov. and gen. nov., sp. nov., nitrate-reducing chemolithoautotrophs of the - isolated from a deep-sea hydrothermal system in the Mid-Okinawa Trough Free

Abstract

Two novel denitrifying bacteria, designated strains MI55-1 and E9I37-1, were isolated from deep-sea hydrothermal vent chimney structures at the Iheya North hydrothermal field in the Mid-Okinawa Trough, Japan. Both isolates were strict chemolithoautotrophs growing by respiratory nitrate reduction with H, forming N as a metabolic product. Oxygen (at low concentrations) could serve as an alternative electron acceptor for growth of the isolates. Growth of strain MI55-1 was observed at temperatures between 40 and 57 °C (optimum, 55 °C; doubling time, 2 h), at pH values between 5·4 and 6·9 (optimum, pH 6·4) and in the presence of between 1·5 and 4·0 % (w/v) NaCl (optimum, 2·5 %). Growth of strain E9I37-1 was observed at temperatures between 28 and 40 °C (optimum, 37 °C; doubling time, 2·5 h), at pH values between 5·6 and 7·6 (optimum, pH 7·0) and in the presence of between 1·5 and 3·5 % (w/v) NaCl (optimum, 3·0 %). The G+C contents of the genomic DNA of strains MI55-1 and E9I37-1 were 29·6 and 35·5 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains MI55-1 and E9I37-1 belonged to groups A and F of the -, but that they had distant phylogenetic relationships with any species, within the phylogenetic groups, that had validly published names (sequence similarities were less than 91 %). On the basis of the physiological and molecular characteristics of the novel isolates, it is proposed that they should each be classified in a novel genus: gen. nov., sp. nov., with MI55-1 (=JCM 12459=DSM 16512) as the type strain, and gen. nov., sp. nov., with E9I37-1 (=JCM 12458=DSM 16511) as the type strain.

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gen. nov., sp. nov. and gen. nov., sp. nov., nitrate-reducing chemolithoautotrophs of the - isolated from a deep-sea hydrothermal system in the Mid-Okinawa Trough
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Nitratiruptor tergarcus gen. nov., sp. nov. and Nitratifractor salsuginis gen. nov., sp. nov., nitrate-reducing chemolithoautotrophs of the ε-Proteobacteria isolated from a deep-sea hydrothermal system in the Mid-Okinawa Trough
Int J Syst Evol Microbiol 55, 925 (2005); https://doi.org/10.1099/ijs.0.63480-0
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