1887

Abstract

A mesophilic, hydrogenotrophic methanogen, strain ML15, was isolated from an aquaculture fish pond near Wang-gong, Taiwan. The cells were irregular cocci, non-motile, 1·5–2·0 μm in diameter and Gram-negative. Cells of strain ML15 lysed easily in the presence of SDS (0·1 g l) and the S-layer protein had an of 138 800. The catabolic substrates utilized by this strain included formate and H/CO, but not acetate, methanol, trimethylamine or secondary alcohols. Growth did not occur in minimal medium, but was observed when yeast extract and tryptone were added. Strain ML15 grew fastest at 37 °C, pH 6·6–7·0 and with 3 % NaCl. Acetate was not required for cell growth. Trace amounts of tungstate promoted cell growth. The G+C contents of DNA of N2F9704 and strain ML15 were 59·1 and 58·4 mol%, respectively. Sequence analysis of the 16S rRNA genes of strain ML15 and selected species revealed similarities of 95–97 %. Based on the data presented here, it is proposed that strain ML15 (=OCM 789=DSM 15483) represents a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63475-0
2005-03-01
2020-12-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550837.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63475-0&mimeType=html&fmt=ahah

References

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: re-evaluation of a unique biological group. Microb Rev 43:260–296
    [Google Scholar]
  2. Bleicher K., Zellner G., Winter J. 1989; Growth of methanogens on cyclopentanol/CO2 and specificity of alcohol dehydrogenase. FEMS Microbiol Lett 59:307–312 [CrossRef]
    [Google Scholar]
  3. Blotevogel K.-H., Gahl-Janßen R., Janssen S., Fischer U., Pilz F., Auling G., Macario A. J. L., Tindall B. J. 1991; Isolation and characterization of a novel mesophilic, fresh-water methanogen from river sediment Methanoculleus oldenburgensis sp. nov. Arch Microbiol 157:54–59
    [Google Scholar]
  4. Boone D. R., Whitman W. B. 1988; Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int J Syst Bacteriol 38:212–219 [CrossRef]
    [Google Scholar]
  5. Boone D. R., Whitman W. B., Rouvière P. 1993; Diversity and taxonomy of methanogens. In Methanogenesis pp  35–80 Edited by Ferry J. G. New York, NY: Chapman & Hall;
    [Google Scholar]
  6. Corder R. E., Hook L. A., Larkin J. M., Frea J. I. 1983; Isolation and characterization of two new methane-producing cocci: Methanogenium olentangyi , sp.nov., and Methanococcus deltae , sp. nov. Arch Microbiol 134:28–32 [CrossRef]
    [Google Scholar]
  7. Devereux R., He S.-H., Doyle C. L., Orkland S., Stahl D. A., LeGall J., Whitman W. B. 1996; Diversity and origin of Desulfovibrio species: phylogenetic definition of a family. J Bacteriol 172:3609–3619
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogenetic inference package), version 3.5c Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  9. Fry N. K., Warwick S., Saunders N. A., Embley T. M. 1991; The use of 16S ribosomal RNA analyses to investigate the phylogeny of the Legionellaceae . J Gen Microbiol 137:1215–1222 [CrossRef]
    [Google Scholar]
  10. Jain M. K., Bhatnagar L., Zeikus J. G. 1988; A taxonomic overview of methanogens. Indian J Microbiol 28:143–177
    [Google Scholar]
  11. Jan R.-L., Wu J., Chaw S.-M., Tsai C.-W., Tsen S.-D. 1999; A novel species of thermoacidophilic archaeon, Sulfolobus yangmingensis sp. nov. Int J Syst Bacteriol 49:1809–1816 [CrossRef]
    [Google Scholar]
  12. Jarrell K. F., Faguy D., Hebert A. M., Kalmokoff M. L. 1992; A general method of isolating high molecular weight DNA from methanogenic archaea (archaebacteria. Can J Microbiol 38:65–68 [CrossRef]
    [Google Scholar]
  13. König H. 1995; Isolation and analysis of cell walls from methanogenic Archaea . In Archaea: a Laboratory Manual , vol. 2, Methanogens pp  315–328 Edited by Sowers K. R., Schreier H. T. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
    [Google Scholar]
  15. Lai M.-C., Chen S.-C. 2001; Methanofollis aquaemaris sp. nov., a methanogen isolated from an aquaculture fish pond. Int J Syst Evol Microbiol 51:1873–1880 [CrossRef]
    [Google Scholar]
  16. Lai M.-C., Shih C.-J. 2001; Characterization of Methanococcus voltaei strain P2F9701a: a new methanogen isolated from estuarine environment. Curr Microbiol 42:432–437 [CrossRef]
    [Google Scholar]
  17. Lai M.-C., Shu C.-M., Chiou M.-S., Hong T.-Y., Chuang M.-J., Hua J. J. 1999; Characterization of Methanosarcina mazei strain N2M9705 isolated from an aquaculture fishpond. Curr Microbiol 39:79–84 [CrossRef]
    [Google Scholar]
  18. Lai M.-C., Chen S.-C., Shu C.-M. 7 other authors 2002; Methanocalculus taiwanensis sp. nov., isolated from an estuarine environment. Int J Syst Evol Microbiol 52:1799–1806 [CrossRef]
    [Google Scholar]
  19. Lai M.-C., Lin C.-C., Yu P.-H., Huang Y.-F., Chen S.-C. 2004; Methanocalculus chunghsingensis sp. nov., isolated from an estuary and a marine fishpond in Taiwan. Int J Syst Evol Microbiol 54:183–189 [CrossRef]
    [Google Scholar]
  20. Maestrojuán G. M., Boone D. R., Xun L., Mah R. A., Zhang L. 1990; Transfer of Methanogenium bourgense , Methanogenium marisnigri , Methanogenium olentangyi , and Methanogenium thermophilicum to the genus Methanoculleus gen. nov., emendation of Methanoculleus marisnigri and Methanogenium and description of new strains of Methanoculleus bourgense and Methanoculleus marisnigri . Int J Syst Bacteriol 40:117–122 [CrossRef]
    [Google Scholar]
  21. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1996; The Ribosomal Database Project (RDP). Nucleic Acids Res 24:82–85 [CrossRef]
    [Google Scholar]
  22. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  23. Ollivier B. M., Mah R. A., Garcia J. L., Boone D. R. 1986; Isolation and characterization of Methanogenium bourgense sp. nov. Int J Syst Bacteriol 36:297–301 [CrossRef]
    [Google Scholar]
  24. Ollivier B., Fardeau M.-L., Cayol J.-L., Magot M., Patel B. K. C., Prensier G., Garcia J.-L. 1998; Methanocalculus halotolerans gen. nov., sp. nov., isolated from an oil-producing well. Int J Syst Bacteriol 48:821–828 [CrossRef]
    [Google Scholar]
  25. Rivard C. J., Smith P. H. 1982; Isolation and characterization of a thermophilic marine methanogenic bacterium, Methanogenium thermophilicum sp. nov. Int J Syst Bacteriol 32:430–436 [CrossRef]
    [Google Scholar]
  26. Romesser J. A., Wolfe R. S., Mayer F., Spiess E., Walther-Mauruschat A. 1979; Methanogenium , a new genus of marine methanogenic bacteria, and characterization of Methanogenium cariaci sp.nov. and Methanogenium marisnigri sp. nov. Arch Microbiol 121:147–153 [CrossRef]
    [Google Scholar]
  27. Rouvière P., Mandelco L., Winker S., Woese C. R. 1992; A detailed phylogeny for the Methanomicrobiales . Syst Appl Microbiol 15:363–371 [CrossRef]
    [Google Scholar]
  28. Sowers K. R., Noll K. M. 1995; Techniques for anaerobic growth. In Archaea: a Laboratory Manual , vol. 2, Methanogens pp  15–48 Edited by Sowers K. R., Schreier H. T. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Widdel F. 1986; Growth of methanogenic bacteria in pure culture with 2-propanol and other alcohols as hydrogen donors. Appl Environ Microbiol 51:1056–1062
    [Google Scholar]
  30. Widdel F., Rouviere P. E., Wolfe R. S. 1988; Classification of secondary alcohol-utilizing methanogens including a new thermophilic isolate. Arch Microbiol 150:477–481 [CrossRef]
    [Google Scholar]
  31. Woese C. R., Kandler O., Wheelis M. L. 1990; Towards a natural system of organisms: proposal for the domains Archaea, Bacteria and Eucarya. Proc Natl Acad Sci U S A 87:4576–4579 [CrossRef]
    [Google Scholar]
  32. Xun L., Boone D. R., Mah R. A. 1989; Deoxyribonucleic acid hybridization study of Methanogenium and Methanocorpusculum species, emendation of the genus Methanocorpusculum , and transfer of Methanogenium aggregans to the genus Methanocorpusculum as Methanocorpusculum aggregans comb. nov. Int J Syst Bacteriol 39:109–111 [CrossRef]
    [Google Scholar]
  33. Zabel H. P., König H., Winter J. 1984; Isolation and characterization of a new coccoid methanogen, Methanogenium tationis spec. nov. from a solfataric field on Mount Tatio. Arch Microbiol 137:308–315 [CrossRef]
    [Google Scholar]
  34. Zellner G., Sleytr U. B., Messner P., Kneifel H., Winter J. 1990; Methanogenium liminatans spec. nov., a new coccoid, mesophilic methanogen able to oxidize secondary alcohols. Arch Microbiol 153:287–293 [CrossRef]
    [Google Scholar]
  35. Zellner G., Conway de Macario A. J. L., Conway de Macario E. 1997; A study of three anaerobic methanogenic bioreactors reveals that syntrophs are diverse and different from reference organisms. FEMS Microbiol Ecol 22:295–301 [CrossRef]
    [Google Scholar]
  36. Zellner G., Messner P., Winter J., Stackebrandt E. 1998; Methanoculleus palmolei sp. nov., an irregularly coccoid methanogen from an anaerobic digester treating wastewater of a palm oil plant in North-Sumatra, Indonesia. Int J Syst Bacteriol 48:1111–1117 [CrossRef]
    [Google Scholar]
  37. Zellner G., Boone D. R., Keswani J., Whitman W. B., Woese C. R., Hagelstein A., Tindall B. J., Stackebrandt E. 1999; Reclassification of Methanogenium tationis and Methanogenium liminatans as Methanofollis tationis gen.nov., comb. nov. and Methanofollis liminatans comb. nov. and description of a new strain of Methanofollis liminatans . Int J Syst Bacteriol 49:247–255 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63475-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63475-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error