1887

Abstract

A novel aromatic-compound-degrading bacterium, strain T3-B9, was isolated from sediment of Taihu Lake, Jiangsu Province, south-east China. This bacterial isolate assimilated several aromatic compounds such as phenol, aniline, nitrobenzene, 4-chloronitrobenzene and phenanthrene. The taxonomy of strain T3-B9 was studied by polyphasic methods. The organism showed a range of phenotypic and chemotaxonomic properties consistent with those of the genus . The 16S rRNA gene sequence similarity of strain T3-B9 to members of the genus ranged from 91·6 to 97·5 %, and this isolate clustered phylogenetically with members of genus . The DNA–DNA relatedness values of strain T3-B9 to the most phylogenetically related species, DSM 12447, ATCC 700278 and ATCC 700280, were 31, 33 and 14 %, respectively. The combined genotypic and phenotypic data show that strain T3-B9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T3-B9 (=AS 1.3432=JCM 12465).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63468-0
2005-05-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/3/ijs551229.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63468-0&mimeType=html&fmt=ahah

References

  1. Balkwill D. L., Drake G. R., Reeves R. H. 7 other authors 1997; Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp. nov., Sphingomonas subterranea sp. nov., and Sphingomonas stygia sp. nov. Int J Syst Bacteriol 47:191–201 [CrossRef]
    [Google Scholar]
  2. Busse H.-J., Kämpfer P., Denner E. B. M. 1999; Chemotaxonomic characterisation of Sphingomonas . J Ind Microbiol Biotechnol 23:242–251 [CrossRef]
    [Google Scholar]
  3. Collins M. D. 1985; Isoprenoid quinone analyses in classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minnikin D. E. Orlando: Academic Press;
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation;
    [Google Scholar]
  6. Fredrickson J. K., Balkwill D. L., Drake G. R., Romine M. F., Ringelberg D. B., White D. C. 1995; Aromatic-degrading Sphingomonas isolates from the deep subsurface. Appl Environ Microbiol 61:1917–1922
    [Google Scholar]
  7. Fujii K., Satomi M., Morita N., Motomura T., Tanaka T., Kikuchi S. 2003; Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. Int J Syst Evol Microbiol 53:47–52 [CrossRef]
    [Google Scholar]
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Hu Y.-T., Zhou P.-J., Zhou Y.-G., Liu Z.-H., Liu S.-J. 2004; Saccharothrix xinjiangensis sp. nov., a pyrene-degrading actinomycete isolated from Tianchi lake, Xinjiang, China. Int J Syst Evol Microbiol 54:2091–2094 [CrossRef]
    [Google Scholar]
  10. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  11. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Denner E. B. M., Meyer S., Moore E. R. B., Busse H.-J. 1997; Classification of “ Pseudomonas azotocolligans ” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47:577–583 [CrossRef]
    [Google Scholar]
  13. Kämpfer P., Witzenberger R., Denner E. B. M., Busse H.-J., Neef A. 2002; Novosphingobium hassiacum sp. nov., a new species isolated from an aerated sewage pond. Syst Appl Microbiol 25:37–45 [CrossRef]
    [Google Scholar]
  14. Karlson U., Rojo F., van Elsas J. D., Moore E. 1995; Genetic and serological evidence for the recognition of four pentachlorophenol-degrading bacterial strains as a species of the genus Sphingomonas . Syst Appl Microbiol 18:539–548 [CrossRef]
    [Google Scholar]
  15. Kim E., Aversano P. J., Romine M. F., Schneider R. P., Zylstra G. J. 1996; Homology between genes for aromatic hydrocarbon degradation in surface and deep-subsurface Sphingomonas strains. Appl Environ Microbiol 62:1467–1470
    [Google Scholar]
  16. Leifson E. 1962; The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium , Flavobacterium , Spirillum , and Pseudomonas . Int Bull Bacteriol Nomencl Taxon 12:161–170
    [Google Scholar]
  17. Liu Z., Yang H., Huang Z., Zhou P., Liu S.-J. 2002; Degradation of aniline by newly isolated, extremely aniline-tolerant Delftia sp. AN3. Appl Microbiol Biotechnol 58:679–682 [CrossRef]
    [Google Scholar]
  18. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  19. Nohynek L. J., Nurmiaho-Lassila E. L., Suhonen E. L., Busse H.-J., Mohammadi M., Hantula J., Rainey F., Salkinoja-Salonen M. S. 1996; Description of chlorophenol-degrading Pseudomonas sp. strains KF1T, KF3, and NKF1 as a new species of the genus Sphingomonas , Sphingomonas subarctica sp. nov.. Int J Syst Bacteriol 46:1042–1055 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Sohn J. H., Kwon K. K., Kang J. H., Jung H.-B., Kim S.-J. 2004; Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54:1483–1487 [CrossRef]
    [Google Scholar]
  22. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43:159–271 [CrossRef]
    [Google Scholar]
  23. Stevens T. O., McKinley J. P., Fredrickson J. K. 1993; Bacteria associated with deep, alkaline, anaerobic groundwaters in southeast Washington. Microb Ecol 25:35–50
    [Google Scholar]
  24. Takeuchi M., Sakane T., Yanagi M., Yamasato K., Hamana K., Yokota A. 1995; Taxonomic study of bacteria isolated from plants: proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp. nov., and Sphingomonas mali sp. nov. Int J Syst Bacteriol 45:334–341 [CrossRef]
    [Google Scholar]
  25. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera,Sphingobium , Novosphingobium and Sphingopyxis , on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  27. Tiirola M. A., Busse H.-J., Kämpfer P., Männistö M. 2005; Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process. Int J Syst Evol Microbiol 55:583–588 [CrossRef]
    [Google Scholar]
  28. Ventosa A., Marquez M. C., Kocur M., Tindall B. J. 1993; Comparative study of “ Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . Int J Syst Bacteriol 43:245–248 [CrossRef]
    [Google Scholar]
  29. Wildes H., Wittich R. M., Timmis K. N., Fortnagel P., Francke W. 1996; Degradation of chlorinated dibenzofurans and dibenzo- p -dioxins by Sphingomonas sp. strain RW1. Appl Environ Microbiol 62:367–371
    [Google Scholar]
  30. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology (English translation of Mikrobiologiia ) 16:176–178
    [Google Scholar]
  31. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. 1990; Proposals of Sphingomonas paucimobilis gen.nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov.,Sphingomonas adhaesiva sp. nov., Sphingomonascapsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34:99–119 [CrossRef]
    [Google Scholar]
  32. Yabuuchi E., Kosako Y., Fujiwara N., Naka T., Matsunaga I., Ogura H., Kobayashi K. 2002; Emendation of the genus Sphingomonas Yabuuchi et al . 1990 and junior objective synonymy of the species of three genera, Sphingobium , Novosphingobium and Sphingopyxis , in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 52:1485–1496 [CrossRef]
    [Google Scholar]
  33. Zhang D., Yang H., Zhang W., Huang Z., Liu S.-J. 2003; Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53:1111–1114 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63468-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63468-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error