One whitish and four pinkish strains of Gram-negative, non-motile, aerobic bacteria were isolated from sea-water and sediment samples collected in Chazhma Bay (Sea of Japan, Pacific Ocean). Analysis of 16S rRNA gene sequences revealed that these strains belonged to the ‘Alphaproteobacteria’, having highest sequence similarity of about 94–97 % with species of the genus Loktanella. None of the strains degraded gelatin, casein, chitin, agar, DNA or starch and they had limited ability to utilize carbon sources. The four pinkish strains, Fg36T, Fg1, Fg116 and Fg117, degraded Tween 80. Sea-water strain R10SW5T grew at 3–6 % NaCl and a temperature range of 8–35 °C, whilst strains Fg36T, Fg1, Fg116 and Fg117 grew at NaCl concentrations of 1–12 % and a temperature range of 4–35 °C. Phosphatidylglycerol (58/79 %), diphosphatidylglycerol (11/6 %) and phosphatidylcholine (28/22 %) were the major phospholipids. The predominant fatty acids were 16 : 0 (12·2/8·6 %) and 18 : 1ω7 (76·6/68·4 %). The DNA G+C content of strain R10SW5T was 59·1 mol% and those of the four pinkish strains ranged from 60·5 to 61·8 mol%. Based on the results of phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, two novel species, Loktanella agnita sp. nov. and Loktanella rosea sp. nov., are proposed. The type strains are R10SW5T (=KMM 3788T=CIP 107883T) and Fg36T (=KMM 6003T=CIP 107851T=LMG 22534T), respectively.
HughR.,
LeifsonE.1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J Bacteriol 66:24–26
IvanovaE. P.,
KiprianovaE. A.,
MikhailovV. V.,
LevanovaF. G.,
GaragulyaA. G.,
GorshkovaN. M.,
YumotoN.,
YoshikawaS.1996; Characterization and identification of marine Alteromonas nigrifaciens strains and emendation of the description. Int J Syst Bacteriol 46:223–228[CrossRef]
IvanovaE. P.,
KiprianovaE. A.,
MikhailovV. V.8 other authors1998; Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48:247–256[CrossRef]
IvanovaE. P.,
RomanenkoL. A.,
ChunJ.7 other authors2000; Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including description of two species, Idiomarina abyssalis sp.nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50:901–907[CrossRef]
IvanovaE. P.,
OnyshchenkoO. M.,
ChristenR.,
LysenkoA. M.,
ZhukovaN. V.,
ShevchenkoL. S.,
KiprianovaE. A.2005; Marinomonas pontica sp. nov., isolated from the Black Sea. Int J Syst Evol Microbiol 55:275–279[CrossRef]
LauS. C. K.,
TsoiM. M. Y.,
LiX.,
PlakhotnikovaI.,
WuM.,
WongP.-K.,
QianP.-Y.2004; Loktanella hongkongensis sp. nov., a novel member of the α - Proteobacteria originating from marine biofilms in Hong Kong waters. Int J Syst Evol Microbiol 54:2281–2284[CrossRef]
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118[CrossRef]
RügerH.-J.,
KrambeckH.-J.1994; Evaluation of the BIOLOG substrate metabolism system for classification of marine bacteria. Syst Appl Microbiol 17:281–288[CrossRef]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–655 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
SöllerR.,
HirschP.,
BlohmD.,
LabrenzM.2000; Differentiation of newly described Antarctic bacterial isolates related to Roseobacter species based on 16S–23S rDNA internal transcribed spacer sequences. Int J Syst Evol Microbiol 50:909–915[CrossRef]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849[CrossRef]
UchinoY.,
HirataA.,
YokotaA.,
SugiyamaJ.1999; In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 68. Int J Syst Bacteriol 49:1–3[CrossRef]
Van TrappenS.,
MergaertJ.,
SwingsJ.2004; Loktanella salsilacus gen. nov., sp. nov., Loktanella fryxellensis sp. nov. and Loktanella vestfoldensis sp. nov. new members of the Rhodobacter group, isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:1263–1269[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.9 other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]