1887

Abstract

A spore-forming, halophilic bacterium, designated strain BH163, was isolated from a salt lake in China. Cells were motile, strictly aerobic rods that contained type A1 peptidoglycan with -diaminopimelic acid as the diagnostic diamino acid. The isolate showed Gram- and catalase-positive reactions and formed a terminal endospore with a swollen sporangium. The major cellular fatty acids were anteiso-C, iso-C, anteiso-C and iso-C. The genomic DNA G+C content of the strain was 41·0 mol%. Comparative analysis of 16S rRNA gene sequences showed that strain BH163 formed a distinct line within the phyletic group classically defined as the genus and was most closely related to the taxa [] DSM 5271 and DSM 13259, with 16S rRNA gene sequence similarities of 95·9 and 94·5 %, respectively. On the basis of physiological and molecular properties, it is proposed that [] DSM 5271 is reclassified in the new genus as gen. nov., comb. nov. Strain BH163 (=KCTC 3916=DSM 16460) was assigned as the type strain of the novel species .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63456-0
2005-09-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/5/ijs551891.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63456-0&mimeType=html&fmt=ahah

References

  1. Ash, C., Farrow, J. A. E., Wallbanks, S. & Collins, M. D. ( 1991; ). Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit ribosomal-RNA sequences. Lett Appl Microbiol 13, 202–206.
    [Google Scholar]
  2. Cole, J. R., Chai, B., Marsh, T. L. & 8 other authors ( 2003; ). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  3. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  4. DeLong, E. F. ( 1992; ). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89, 5685–5689.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 2002; ). phylip (Phylogeny Inference Package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, WA, USA.
  7. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  8. Fritze, D. ( 1996; ). Bacillus haloalkaliphilus sp. nov. Int J Syst Bacteriol 46, 98–101.[CrossRef]
    [Google Scholar]
  9. Hao, M. V., Kocur, M. & Komagata, K. ( 1984; ). Marinococcus gen. nov., a new genus for motile cocci with meso-diaminopimelic acid in the cell wall; and Marinococcus albus sp. nov. and Marinococcus halophilus (Novitsky and Kushner) comb. nov. J Gen Appl Microbiol 30, 449–459.[CrossRef]
    [Google Scholar]
  10. Heyndrickx, M., Lebbe, L., Kersters, K., De Vos, P., Forsyth, G. & Logan, N. A. ( 1999; ). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48, 99–106.
    [Google Scholar]
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–208.
    [Google Scholar]
  13. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  14. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  15. Nielsen, P., Rainey, F. A., Outtrup, H., Priest, F. G. & Fritze, D. ( 1994; ). Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus. FEMS Microbiol Lett 117, 61–66.[CrossRef]
    [Google Scholar]
  16. Oren, A. ( 2002; ). Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol 28, 56–63.[CrossRef]
    [Google Scholar]
  17. Ren, P. -G. & Zhou, P. -J. ( 2005; ). Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 95–99.[CrossRef]
    [Google Scholar]
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  20. Schlesner, H., Lawson, P. A., Collins, M. D., Weiss, N., Wehmeyer, U., Völker, H. & Thomm, M. ( 2001; ). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51, 425–431.
    [Google Scholar]
  21. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  22. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  23. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K.-H. ( 1996; ). Halobacillus gen. nov., with description of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophila comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef]
    [Google Scholar]
  24. Stackebrandt, E. & Liesack, W. ( 1993; ). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 152–189. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  25. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  27. Ventosa, A., Garcia, M. T., Kamekura, M., Onishi, H. & Ruiz-Berraquero, F. ( 1989; ). Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Syst Appl Microbiol 12, 162–165.[CrossRef]
    [Google Scholar]
  28. Wainø, M., Tindall, B. J., Schumann, P. & Ingvorsen, K. ( 1999; ). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49, 821–831.[CrossRef]
    [Google Scholar]
  29. Yoon, J. H., Weiss, N., Lee, K. C., Lee, I. S., Kang, K. H. & Park, Y. H. ( 2001; ). Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen nov., comb. nov. Int J Syst Evol Microbiol 51, 2087–2093.[CrossRef]
    [Google Scholar]
  30. Yoon, J. H., Oh, T. K. & Park, Y. H. ( 2004; ). Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54, 2163–2167.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63456-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63456-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1891 - 1896

[PDF](112 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error