1887

Abstract

Four bacterial strains isolated from the human oral cavity, PPPA19, PPPA21, PPPA28 and PPPA30, were characterized by determining phenotypic and biochemical features, cellular fatty acid profiles and the phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that each of the isolates was a member of the genus . These strains were related to with about 95 % similarity. The strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. However, the cells of these strains were often cocci (coccobacilli), depending on the cultivation time. Colonies of different sizes were detected on Eggerth Gagnon agar plates for these strains. The cells forming large colonies were cocci, whereas those forming small colonies were cocci and rods. However, 16S rRNA gene sequence comparison of colonies of different sizes revealed that only a single organism was present. Although these strains had phenotypic characteristics that were similar to those of JCM 8528, they could be differentiated from JCM 8528 by aesculin hydrolysis and -cellobiose fermentation in API 20A tests. DNA–DNA hybridization experiments revealed the genomic distinction of these four strains with respect to JCM 8528. On the basis of these data, a novel species, sp. nov., is proposed, with PPPA21 (=JCM 12541=DSM 16608) as the type strain.

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2005-03-01
2019-10-21
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Photomicrographs of Gram-stained cells from 2-day-old EG agar cultures of strains PPPA21 (left) and PPPA19 (right). Bars, 10 µm.

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Photomicrographs of Gram-stained cells from 2-day-old EG agar cultures of strains PPPA21 (left) and PPPA19 (right). Bars, 10 µm.

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Extended phylogenetic tree showing the position of sp. nov. within the genus . [PDF](16 KB)

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[PDF file of Tables A-D](31 KB)

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