1887

Abstract

Phylogenetic and phenotypic analysis of cultivable marine bacteria isolated from laboratory cultures of two paralytic shellfish toxin-producing dinoflagellates, and , showed the presence of a novel group of Gram-negative, aerobic, moderately halophilic and hydrocarbon-degrading bacteria, related to the genus . The strains, designated DG893, DG1136 and ATAM407-13, grew optimally in media with 3–6 % NaCl and at 25–30 °C, and all could utilize n-hexadecane and n-tetradecane as the sole carbon source. The strains had a 16S rRNA gene sequence similarity of 94·2–94·3 % to ATCC 27132, and a similarity of 97·5–97·8 % to the closest phylogenetically related type strain, DSM 16070. DNA–DNA hybridization levels to and other type strains were ⩽42 %, while DNA–DNA reassociation values among DG893, DG1136 and ATAM407-13 were ⩾83 %. The DNA G+C content was 54–55 mol% and the major isoprenoid quinone was ubiquinone-9. On the basis of phenotypic, chemotaxonomic, DNA–DNA hybridization and phylogenetic analysis, it is proposed that these three strains represent a novel species, sp. nov. The type strain is DG893 (=DSM 16394=NCIMB 14009).

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2006-03-01
2019-10-14
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References

  1. Beveridge, T. J., Popkin, T. J. & Cole, R. M. ( 1994; ). Electron microscopy. In Methods for General and Molecular Bacteriology, pp. 42–71. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  2. Bowman, J. P., McCammon, S. A., Brown, J. L. & McMeekin, T. A. ( 1998; ). Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 48, 1213–1222.[CrossRef]
    [Google Scholar]
  3. Cole, J. R., Chai, B., Marsh, T. L. & 8 other authors ( 2003; ). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  4. Gallacher, S., Flynn, K. J., Franco, J. M., Brueggemann, E. E. & Hines, H. B. ( 1997; ). Evidence for production of paralytic shellfish toxins by bacteria associated with Alexandrium spp. (Dinophyta) in culture. Appl Environ Microbiol 63, 239–245.
    [Google Scholar]
  5. Gauthier, M. J., Lafay, B., Christen, R., Fernandez, L., Acquaviva, M., Bonin, P. & Bertrand, J.-C. ( 1992; ). Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42, 568–576.[CrossRef]
    [Google Scholar]
  6. Gorshkova, N. M., Ivanova, E. P., Sergeev, A. F., Zhukova, N. V., Alexeeva, Y., Wright, J. P., Nicolau, D. V., Mikhailov, V. V. & Christen, R. ( 2003; ). Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan. Int J Syst Evol Microbiol 53, 2073–2078.[CrossRef]
    [Google Scholar]
  7. Green, D. H., Llewellyn, L. E., Negri, A. P., Blackburn, S. I. & Bolch, C. J. S. ( 2004; ). Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum. FEMS Microbiol Ecol 47, 345–357.[CrossRef]
    [Google Scholar]
  8. Hold, G. L., Smith, E. A., Rappe, M. S. & 7 other authors ( 2001; ). Characterisation of bacterial communities associated with toxic and non-toxic dinofagellates: Alexandrium spp. and Scrippsiella trochoidea. FEMS Microbiol Ecol 37, 161–173.[CrossRef]
    [Google Scholar]
  9. Huu, N. B., Denner, E. B. M., Dang, T. C. H., Wanner, G. & Stan-Lotter, H. ( 1999; ). Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49, 367–375.[CrossRef]
    [Google Scholar]
  10. Kodama, M., Ogata, T., Sakamoto, S., Sato, S., Honda, T. & Miwatani, T. ( 1990; ). Production of paralytic shellfish toxins by a bacterium Moraxella sp. isolated from Protogonyaulax tamarensis. Toxicon 28, 707–714.[CrossRef]
    [Google Scholar]
  11. Komagata, K. & Suzuki, K. ( 1987; ). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–203.
    [Google Scholar]
  12. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  13. Martín, S., Márquez, M. C., Sánchez-Porro, C., Mellado, E., Arahal, D. R. & Ventosa, A. ( 2003; ). Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity. Int J Syst Evol Microbiol 53, 1383–1387.[CrossRef]
    [Google Scholar]
  14. Romanenko, L. A., Schumann, P., Rohde, M., Zhukova, N. V., Mikhailov, V. V. & Stackebrandt, E. ( 2005; ). Marinobacter bryozoorum sp. nov. and Marinobacter sediminum sp. nov., novel bacteria from the marine environment. Int J Syst Evol Microbiol 55, 143–148.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Newark, DE: MIDI Inc.
  17. Shieh, W. Y., Jean, W. D., Lin, Y. T. & Tseng, M. ( 2003; ). Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 49, 244–252.[CrossRef]
    [Google Scholar]
  18. Shivaji, S., Gupta, P., Chaturvedi, P., Suresh, K. & Delille, D. ( 2005; ). Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 55, 1453–1456.[CrossRef]
    [Google Scholar]
  19. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  20. Smith, E. A., Mackintosh, F. H., Grant, F. & Gallacher, S. ( 2002; ). Sodium channel blocking (SCB) activity and transformation of paralytic shellfish toxins (PST) by dinoflagellate-associated bacteria. Aquat Microb Ecol 29, 1–9.[CrossRef]
    [Google Scholar]
  21. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  22. Swofford, D. L. ( 2001; ). paup Phylogenetic Analysis Using Parsimony and other Methods, 4th edn. Sunderland, MA: Sinauer Associates.
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  24. Yakimov, M. M., Golyshin, P. N., Lang, S., Moore, E. R. B., Abraham, W.-R., Lünsdorf, H. & Timmis, K. N. ( 1998; ). Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48, 339–348.[CrossRef]
    [Google Scholar]
  25. Yoon, J. H., Yeo, S. H., Kim, I. G. & Oh, T. K. ( 2004; ). Marinobacter flavimaris sp. nov. and Marinobacter daepoensis sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54, 1799–1803.[CrossRef]
    [Google Scholar]
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