1887

Abstract

A Gram-negative, slightly halophilic, non-pigmented, strictly aerobic, chemo-organotrophic bacterium was isolated from Mediterranean sea water off the Spanish coast near Valencia. This strain was poorly reactive, being unable to grow in most carbon sources analysed in minimal medium. However, good growth was observed when more complex media and longer incubation times were used. Phylogenetic analysis based on an almost complete 16S rRNA gene sequence placed strain 2SM4 within the group, in the vicinity of uncultured bacteria described as gall symbionts of several species of the red alga . Sequence similarity values between strain 2SM4 and the closest neighbouring species were below 95·0 %. The cellular fatty acid composition of the Mediterranean strain confirmed its position within the ‘’, sharing 18 : 17 as the major cellular fatty acid. The phylogenetic distance from any taxon with a validly published name and also a number of distinguishing features support the designation of strain 2SM4 as representing a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is 2SM4 (=CECT 5292=DSM 16309=CIP 108404=CCUG 49433).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63442-0
2005-03-01
2019-09-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550631.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63442-0&mimeType=html&fmt=ahah

References

  1. Ashen, J. B. & Goff, L. J. ( 1996; ). Molecular identification of a bacterium associated with gall formation in the marine red alga Prionitis lanceolata. J Phycol 32, 286–297.[CrossRef]
    [Google Scholar]
  2. Ashen, J. B. & Goff, L. J. ( 1998; ). Galls on the marine red alga Prionitis lanceolata (Halymeniaceae): specific induction and subsequent development of an algal-bacterial symbiosis. Am J Bot 85, 1710–1721.[CrossRef]
    [Google Scholar]
  3. Ashen, J. B. & Goff, L. J. ( 2000; ). Molecular and ecological evidence for species specificity and coevolution in a group of marine algal–bacterial symbioses. Appl Environ Microbiol 66, 3024–3030.[CrossRef]
    [Google Scholar]
  4. Baumann, P. & Baumann, L. ( 1981; ). The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes. In The Prokaryotes, vol. II, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. Schlegel. Berlin: Springer.
  5. Benlloch, S., Rodríguez-Valera, F. & Martinez-Murcia, A. J. ( 1995; ). Bacterial diversity in two coastal lagoons deduced from 16S rDNA PCR amplification and partial sequencing. FEMS Microbiol Ecol 18, 267–279.[CrossRef]
    [Google Scholar]
  6. Bowman, J. P., McCammon, S. A., Brown, M. V., Nichols, D. S. & McMeekin, T. A. ( 1997; ). Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63, 3068–3078.
    [Google Scholar]
  7. Garrity, G. M., Bell, J. A. & Lilburn, T. G. ( 2003; ). Taxonomic outline of the procaryotes. In Bergey's Manual of Systematic Bacteriology, 2nd edn, release 4.0. New York: Springer. doi:10.1007/bergeysoutline200310
  8. Gonzalez, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B. ( 1997; ). Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. Int J Syst Bacteriol 47, 773–780.[CrossRef]
    [Google Scholar]
  9. Gonzalez, J. M., Kiene, R. P. & Moran, M. A. ( 1999; ). Transformation of sulfur compounds by an abundant lineage of marine bacteria in the α-subclass of the class Proteobacteria. Appl Environ Microbiol 65, 3810–3819.
    [Google Scholar]
  10. Gonzalez, J. M., Simo, R., Massana, R., Covert, J. S., Casamayor, E. O., Pedros-Alio, C. & Moran, M. A. ( 2000; ). Bacterial community structure associated with a dimethylsulfoniopropionate-producing North Atlantic algal bloom. Appl Environ Microbiol 66, 4237–4246.[CrossRef]
    [Google Scholar]
  11. Gosink, J. J. & Staley, J. T. ( 1995; ). Biodiversity of gas vacuolate bacteria from Antarctic Sea ice and water. Appl Environ Microbiol 61, 3486–3489.
    [Google Scholar]
  12. Heimbrook, M. E., Wang, W. L. L. & Campbell, G. ( 1989; ). Staining bacterial flagella easily. J Clin Microbiol 27, 2612–2615.
    [Google Scholar]
  13. Ivanova, E. P., Gorshkova, N. M., Sawabe, T. & 8 other authors ( 2004; ). Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish (Stellaster equestris) and sea grass (Zostera marina). Int J Syst Evol Microbiol 54, 475–480.[CrossRef]
    [Google Scholar]
  14. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  15. Labrenz, M., Tindall, B. J., Lawson, P. A., Collins, M. D., Schumann, P. & Hirsch, P. ( 2000; ). Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., α-3-Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake. Int J Syst Evol Microbiol 50, 303–313.[CrossRef]
    [Google Scholar]
  16. Ludwig, W., Strunk, O., Klugbauer, S., Klugbauer, N., Weizenegger, M., Neumaier, J., Bachleitner, M. & Schleifer, K.-H. ( 1998; ). Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568.[CrossRef]
    [Google Scholar]
  17. Ludwig, W., Strunk, O., Westram, R. & 29 other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  18. Macián, M. C., Ludwig, W., Schleifer, K. H., Garay, E. & Pujalte, M. J. ( 2001; ). Thalassomonas viridans gen. nov., sp. nov., a novel marine γ-proteobacterium. Int J Syst Evol Microbiol 51, 1283–1289.
    [Google Scholar]
  19. Macián, M. C., Arahal, D. R., Garay, E., Ludwig, W., Schleifer, K. H. & Pujalte, M. J. ( 2005; ). Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine α-proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55, 105–110.[CrossRef]
    [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  21. Ortigosa, M., Garay, E. & Pujalte, M. J. ( 1994; ). Numerical taxonomy of aerobic, Gram-negative bacteria associated with oysters and surrounding seawater of the Mediterranean coast. Syst Appl Microbiol 17, 589–600.
    [Google Scholar]
  22. Pukall, R., Buntefuß, D., Frühling, A., Rohde, M., Kroppenstedt, R. M., Burghardt, J., Lebaron, P., Bernard, L. & Stackebrandt, E. ( 1999; ). Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria. Int J Syst Bacteriol 49, 513–519.[CrossRef]
    [Google Scholar]
  23. Rappé, M. S., Connon, S. A., Vergin, K. L. & Giovannoni, S. J. ( 2002; ). Cultivation of the ubiquitous SAR11 marine bacterioplankton clade. Nature 418, 630–633.[CrossRef]
    [Google Scholar]
  24. Wagner-Döbler, I., Rheims, H., Felske, A., El-Ghezal, A., Flade-Schröder, D., Laatsch, H., Lang, S., Pukall, R. & Tindall, B. J. ( 2004; ). Oceanibulbus indolifex gen. nov., sp. nov., a North Sea alphaproteobacterium that produces bioactive metabolites. Int J Syst Evol Microbiol 54, 1177–1184.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63442-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63442-0
Loading

Data & Media loading...

Transmission and scanning electron micrographs showing the characteristic variations in cell morphology of strain 2SM4 from coccoid cells to elongated rods, including many tear-shaped cells. [PDF](611 KB)

PDF

Biolog oxidations of gen. nov., sp. nov. and related species. [PDF](26 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error