1887

Abstract

A Gram-negative bacterium, designated LA33B, was isolated from water collected from a hypersaline lake on uninhabited Laysan Atoll in the Northwestern Hawaiian Islands. Cells of strain LA33B are motile, straight rods that grow between 4 and 45 °C and in media containing 1–17·5 % (w/v) NaCl. The strain oxidizes carbohydrates, nucleosides, amino acids and organic acids presented as sole carbon sources and constitutive lipolytic and proteolytic enzymes are expressed. Over 75 % of the fatty acid pool is -11-octadecenoic acid (18 : 17). Comparative sequence analysis of the 16S rRNA gene indicates that the strain forms a new lineage in the -2 subclass of the , with the closest recognized strains being NCIMB 2208 and JCM 10543, with which it shares 94–95 % sequence similarity. Strain LA33B differs phenotypically from extant and species, however, in that it is a moderate thermophile, it requires NaCl and tolerates higher NaCl concentrations and it does not express -galactosidase or oxidize glycerol. On the basis of genotypic data and phenotypic characteristics, we propose that strain LA33B does not belong to the genera or and that it represents the type species of a new genus, . Strain LA33B (=ATCC BAA-994=CIP 108449) is proposed as the type strain of the type species of this genus, with the name gen. nov., sp. nov.

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2006-03-01
2020-01-17
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vol. , part 3, pp. 563 - 567

Fatty acids in whole cells of LA33B .

SEM of whole cells of LA33B .

A chart showing growth of LA33B in 50 % marine broth 2216E with different concentrations (% w/v) of NaCl.

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