1887

Abstract

Two thermophilic, spore-forming strains, TM1 and TM5, were isolated from deep-sea sediment (4000 m below sea level) of the Ayu Trough in the western Pacific Ocean. Cells of the two strains were Gram-positive, motile and rod-shaped. Their spores were ellipsoidal, subterminal to terminal and occurred in swollen sporangia. The two strains grew at temperatures up to 65 °C and in the pH range 6·5–9·0. The NaCl concentration for optimal growth was 3·0 % (w/v) and growth was inhibited by 5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains TM1 and TM5 belonged to the genus , and that strain TM1 was most closely related to DSM 15084 (96·7 %), DSM 4216 (96·1 %), NCIMB 13113 (95·8 %) and DSM 3670 (95·7 %). Between the 16S rRNA gene sequences of strains TM1 and TM5 there were only three nucleotide differences, implying that the two strains were of the same species. The cellular fatty acid profiles of the two strains were also very similar, with iso-C, iso-C, C, iso-C and anteiso-C as the major components. The G+C content of strain TM1 was 38·7 %. On the basis of phenotypic and molecular data, strains TM1 and TM5 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TM1 (=KCTC 10634=JCM 12523).

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2005-05-01
2019-10-22
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vol. , part 3, pp. 1211–1215

A table giving details of the cellular fatty acid compositions of strains TM1 and TM5 and related thermophilic species is available to download. [PDF](80KB)



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