1887

Abstract

Strains of a Gram-positive bacterium were isolated from the Irish smear-ripened cheese Gubbeen, and assigned to a new species, , in 2001. During a further study on the same cheese, no additional isolates from this species could be found. Instead, multiple isolates of its nearest phylogenetic neighbour, , were found. A first screening with rep-PCR and SDS-PAGE pointed to a similarity between and . Following this peculiar result, attempts were made to collect all type strains deposited at different culture collections and all strains described by Brennan . [ (2001) , 843–852] . Subsequently, 16S rRNA gene sequencing and DNA–DNA hybridizations were performed. All strains had a 16S rRNA gene sequence similarity of at least 99·5 % with and the DNA–DNA relatedness was 95 %. On the basis of these results, it is concluded that is a later heterotypic synonym of .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63420-0
2005-05-01
2021-03-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/3/ijs551129.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63420-0&mimeType=html&fmt=ahah

References

  1. Brennan N. M., Brown R., Goodfellow M., Ward A. C., Beresford T. P., Simpson P. J., Fox P. F., Cogan T. M. 2001; Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese. Int J Syst Evol Microbiol 51:843–852 [CrossRef]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Kersters K., De Ley K. 1975; Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patters. J Gen Microbiol 87:333–342 [CrossRef]
    [Google Scholar]
  4. Logan N. A., Lebbe L., Hoste B. 7 other authors 2000; Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50:1741–1753
    [Google Scholar]
  5. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  6. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester, UK: Wiley;
    [Google Scholar]
  7. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  8. Versalovic J., Schneider M., de Bruijn F. J., Lupski J. R. 1994; Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40
    [Google Scholar]
  9. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  10. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63420-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63420-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error