1887

Abstract

Strains of a Gram-positive bacterium were isolated from the Irish smear-ripened cheese Gubbeen, and assigned to a new species, , in 2001. During a further study on the same cheese, no additional isolates from this species could be found. Instead, multiple isolates of its nearest phylogenetic neighbour, , were found. A first screening with rep-PCR and SDS-PAGE pointed to a similarity between and . Following this peculiar result, attempts were made to collect all type strains deposited at different culture collections and all strains described by Brennan . [ (2001) , 843–852] . Subsequently, 16S rRNA gene sequencing and DNA–DNA hybridizations were performed. All strains had a 16S rRNA gene sequence similarity of at least 99·5 % with and the DNA–DNA relatedness was 95 %. On the basis of these results, it is concluded that is a later heterotypic synonym of .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63420-0
2005-05-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/3/ijs551129.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63420-0&mimeType=html&fmt=ahah

References

  1. Brennan, N. M., Brown, R., Goodfellow, M., Ward, A. C., Beresford, T. P., Simpson, P. J., Fox, P. F. & Cogan, T. M. ( 2001; ). Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese. Int J Syst Evol Microbiol 51, 843–852.[CrossRef]
    [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Kersters, K. & De Ley, K. ( 1975; ). Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patters. J Gen Microbiol 87, 333–342.[CrossRef]
    [Google Scholar]
  4. Logan, N. A., Lebbe, L., Hoste, B. & 7 other authors ( 2000; ). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50, 1741–1753.
    [Google Scholar]
  5. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  6. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester, UK: Wiley.
  7. Stackebrandt, E., Frederiksen, W., Garrity, G. M. & 10 other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  8. Versalovic, J., Schneider, M., de Bruijn, F. J. & Lupski, J. R. ( 1994; ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40.
    [Google Scholar]
  9. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  10. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63420-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63420-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error