1887

Abstract

Strain TL 2 was isolated on mineral medium with thiosulfate from a near-shore sulfidic hydrothermal area in Matupi Harbour on the island of New Britain, Papua New Guinea. The cells varied from long filaments with swollen ends, often aggregated, to short rods, depending on the growth conditions. The bacterium was obligately aerobic and grew autotrophically with thiosulfate as energy source or heterotrophically with organic acids and sugars. In thiosulfate-limited continuous culture, and for autotrophic growth were 0·1 h and 3 g protein mol, respectively. From the various reduced sulfur compounds tested, only thiosulfate and sulfide supported active respiration. Inorganic carbon was assimilated via the Calvin cycle. Presence of the ‘green’-type of form I RubisCO gene was detected. Growth was possible from 15 to 47 °C with an optimum at 35 °C, pH 6·5–8·5 with an optimum at pH 8·0, and between 10 and 90 g NaCl l with an optimum at 35 g l. Phylogenetic analysis based on 16S rRNA and gene sequences demonstrated that strain TL 2 forms a separate lineage within the alpha-3 subdivision of the , distantly related to the genera and . On the basis of these results, a novel genus and species, gen. nov., sp. nov., is proposed to accommodate strain TL 2 (=DSM 10166=UNIQEM 229).

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2005-05-01
2024-03-29
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References

  1. Albuquerque L., Santos J., Travassos P., Nobre M. F., Rainey F. A., Wait R., Empadinhas N., Silva M. T., da Costa M. S. 2002; Albidovulum inexpectatum gen. nov., sp. nov. a nonphotosynthetic and slightly thermophilic bacterium from a marine hot spring that is very closely related to members of the photosynthetic genus Rhodovulum . Appl Environ Microbiol 68:4266–4273 [CrossRef]
    [Google Scholar]
  2. Beudecker R. F., Cannon G. C., Kuenen J. G., Shively J. M. 1980; Relations between d-ribulose-1,5-bisphosphate carboxylase, carboxysomes and CO2-fixing capacity in the obligate chemolithotroph Thiobacillus neapolitanus grown under different limitations in the chemostat. Arch Microbiol 124:185–189
    [Google Scholar]
  3. Beudecker R. F., Gottschal J. G., Kuenen J. G. 1982; Reactivity versus flexibility in thiobacilli. Antonie van Leeuwenhoek 48:39–51 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1989; phylip - Phylogenetic Inference Package version 3.2. Cladistics 5:164–166
    [Google Scholar]
  5. Ferguson J., Lambert I. B. 1972; Volcanic exhalations and metal enrichments at Matupi Harbour, New Britain, T.P.N.G. Econ Geol 67:25–37 [CrossRef]
    [Google Scholar]
  6. Gottschal J. C., Kuenen J. G. 1980; Selective enrichment of facultatively chemolithotrophic thiobacilli and related organisms in the chemostat. FEMS Microbiol Lett 7:241–247 [CrossRef]
    [Google Scholar]
  7. Guay R., Silver M. 1975; Thiobacillus acidophilus sp. nov.; isolation and some physiological characteristics. Can J Microbiol 21:281–288 [CrossRef]
    [Google Scholar]
  8. Kelly D. P. 1989; Physiology and biochemistry of unicellular sulfur bacteria. In Autotrophic Bacteria pp 193–217 Edited by Schlegel H. G., Bowien B. Berlin: Springer;
    [Google Scholar]
  9. Kelly D. P., Wood A. P. 1994; Enzymes involved in microbiological oxidation of thiosulfate and polythionates. Methods Enzymol 243:501–520
    [Google Scholar]
  10. Kelly D. P., Chambers L. A., Trudinger P. A. 1969; Cyanolysis and spectrophotometric estimation of trithionate in mixture with thiosulfate and tetrathionate. Anal Chem 41:898–901 [CrossRef]
    [Google Scholar]
  11. Kuenen J. G. 1989; Comparative ecophysiology of the nonphototrophic sulfide-oxidizing bacteria. In Microbial Mats: Physiological Ecology of Benthic Microbial Communities pp  349–365 Edited by Cohen I., Rosenberg E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Paoli G. C., Soyer F., Shively J., Tabita F. R. 1998; Rhodobacter capsulatus genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase ( cbbLS ) and neighbouring genes were acquired by a horizontal gene transfer. Microbiology 144:219–227 [CrossRef]
    [Google Scholar]
  13. Pfennig N., Lippert K. D. 1966; Über das Vitamin B12 – bedürfnis phototropher Schwefel bacterien. Arch Microbiol 55:245–256 (in German
    [Google Scholar]
  14. Sorokin D. Yu. 1991; Oxidation of reduced sulfur compounds in volcanically active regions of the Plenty Bay (New Zealand) and Matupi Harbour (New Britain, Papua New Guinea). Izv Akad Nauk S S S R Ser Biol376–387 (in Russian
    [Google Scholar]
  15. Sorokin D. Yu. 1992; Catenococcus thiocyclus gen. nov., sp. nov. – a new facultatively anaerobic bacterium from a near-shore sulphidic hydrothermal area. J Gen Microbiol 138:2287–2292 [CrossRef]
    [Google Scholar]
  16. Sorokin D. Y., Robertson L. A., Kuenen J. G. 1996; Sulfur cycling in Catenococcus thiocyclus . FEMS Microbiol Ecol 19:117–126
    [Google Scholar]
  17. Sorokin D. Yu., Tourova T. P., Sjollema K. A., Kuenen G. J. 2003; Thialkalivibrio nitratireducens sp. nov., a nitrate-reducing member of an autotrophic denitrifying consortium from a soda lake. Int J Syst Evol Microbiol 53:1779–1783 [CrossRef]
    [Google Scholar]
  18. Spiridonova E. M., Berg I. A., Kolganova T. V., Ivanovsky R. N., Kuznetsov B. B., Tourova T. P. 2004; A system of oligonucleotide primers for the amplification of the ribulose-1,5-bisphosphate carboxylase/oxygenase genes of different taxonomic groups of bacteria. Mikrobiologiia 73:316–325 (in Russian
    [Google Scholar]
  19. Trüper H. G., Schlegel H. G. 1964; Sulphur metabolism in Thiorhodaceae . 1. Quantitative measurements on growing cells of Chromatium okenii . Antonie van Leeuwenhoek 30:225–238 [CrossRef]
    [Google Scholar]
  20. Uchino Y., Yokota A. 2003; “Green-like” and “red-like” RuBisCO cbbL genes in Rhodobacter azotoformans . Mol Biol Evol 20:821–830 [CrossRef]
    [Google Scholar]
  21. Uchino Y., Hamada T., Yokota A. 2002; Proposal of Pseudorhodobacter ferrugineus gen. nov., comb. nov., for a non-photosynthetic marine bacterium, Agrobacterium ferrugineum , related to the genus Rhodobacter . J Gen Appl Microbiol 48:309–319 [CrossRef]
    [Google Scholar]
  22. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  23. Yurkov V. V., Beatty T. 1998; Aerobic anoxygenic phototrophic bacteria. Microbiol Mol Biol Rev 62:695–724
    [Google Scholar]
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