1887

Abstract

A marine bacterial strain, designated FR1050, was isolated from a sediment sample of getbol (Korean tidal flat). Phylogenetic investigations based on 16S rRNA gene sequence analysis showed that the isolate formed a robust monophyletic clade with within the -. Sequence similarity between strain FR1050 and the type strain of was 94·7 %. Cells were Gram-negative, aerobic, rod-shaped, motile and halophilic; optimum growth occurred at sea salt concentrations of 4–6 %. The major fatty acids were C 9 (39·0 %) and C (18·1 %). The DNA G+C content was 44 mol%. The polyphasic data obtained showed that strain FR1050 is affiliated to the genus but represents a novel species for which the name sp. nov. is proposed. The type strain is FR1050 (=KCTC 12277=JCM 12486).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63411-0
2005-03-01
2019-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550681.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63411-0&mimeType=html&fmt=ahah

References

  1. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  2. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1993; ). phylip (Phylogeny Inference Package), version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, USA.
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  7. Gutell, R. R. ( 1994; ). Collection of small subunit (16S- and 16S-like) ribosomal RNA structure: 1994. Nucleic Acids Res 22, 3502–3507.[CrossRef]
    [Google Scholar]
  8. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  9. Kovacs, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703.
    [Google Scholar]
  10. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by M. Goodfellow. Chichester: Wiley.
  11. Lee, H. K., Chun, J., Moon, E. Y., Ko, S. H., Lee, D. S., Lee, H. S. & Bae, K. S. ( 2001; ). Hahella chejuensis gen. nov., sp. nov., an extracellular-polysaccharide-producing marine bacterium. Int J Syst Evol Microbiol 51, 661–666.
    [Google Scholar]
  12. Lyman, J. & Fleming, R. H. ( 1940; ). Composition of sea water. J Mar Res 3, 134–146.
    [Google Scholar]
  13. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Swofford, D. L. ( 1998; ). paup – Phylogenetic Analysis Using Parsimony, version 4. Sunderland, MA: Sinauer Associates.
  16. Zobell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63411-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63411-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error