1887

Abstract

An obligately methanotrophic bacterial strain, LW14, isolated from the sediment of Lake Washington, Seattle, USA, is described taxonomically. The isolate is an aerobic, Gram-negative, non-motile bacterium capable of growth on methane, and possesses type I intracytoplasmic membranes (i.e. it is a type I methanotroph). The strain possesses particulate methane monooxygenase (MMO) and has no soluble MMO. Formaldehyde is assimilated via the ribulose monophosphate cycle. The isolate grows within a pH range of 4–8, with the optimum between pH 5·5 and 6·5. The cellular fatty acid profile is dominated by C 18, C 7 and C 5 fatty acids. The DNA G+C content is 53·3±0·4 mol%. On the basis of sequence analysis of the 16S rRNA gene, isolate LW14 is related most closely to representatives of the genus . However, DNA–DNA hybridization analysis reveals only a distant relationship between isolate LW14 and the previously described species. On the basis of its phenotypic and genotypic characteristics, LW14 represents a novel species of the genus , for which the name sp. nov. is proposed, with LW14 (=ATCC BAA-1047=JCM 13284) as the type strain.

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2005-11-01
2020-09-28
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