1887

Abstract

Two strains were isolated from tuff, a volcanic rock that forms the walls of the Roman Catacombs of Saint Callixtus in Rome, Italy. A polyphasic approach using nutritional and physiological tests, reactions to antibiotics, fatty acid profiles, DNA base ratios, DNA–DNA reassociation and 16S rRNA gene sequence comparisons showed that the two isolates belong to a novel species within the genus . The species sp. nov. is proposed. The type strain is CSC19 (=CECT 5680=LMG 22520).

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2005-07-01
2024-10-04
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  3. Bernaerts M. J., De Ley J. 1963; A biochemical test for crown gall bacteria. Nature 197:406–407
    [Google Scholar]
  4. Boquet E., Bordonat A., Ramos Cormenzana A. 1973; Production of calcite crystals by soil bacteria is a general phenomenon. Nature 246:527–528 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press;
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  7. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [CrossRef]
    [Google Scholar]
  8. Gonzalez J. M., Saiz-Jimenez C. 2005; A simple fluorimetric method for the estimation of DNA–DNA relatedness between closely related microorganisms by thermal denaturation temperatures. Extremophiles 9:75–79 [CrossRef]
    [Google Scholar]
  9. Gonzalez J. M., Jurado V., Laiz L., Zimmermann J., Hermosin B., Saiz-Jimenez C. 2004; Pectinatus portalensis nov. sp., a relatively fast-growing, coccoidal, novel Pectinatus species isolated from a wastewater treatment plant. Antonie van Leeuwenhoek 86:241–247 [CrossRef]
    [Google Scholar]
  10. Halebian S., Harris B., Finegold S. M., Rolfe R. D. 1981; Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria. J Clin Microbiol 13:444–448
    [Google Scholar]
  11. Knösel D. 1962; Prüfung von bakterien auf fähigkeit zur sternbildung. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg II. Abt 116:79–100 (in German
    [Google Scholar]
  12. Knösel D. 1984a; Genus IV Phyllobacterium ( ex Knösel 1962) nom. rev. ( Phyllobacterium Knösel 1962, 96). In Bergey's Manual of Systematic Bacteriology vol 1 pp  254–256 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  13. Knösel D. 1984b; Genus Phyllobacterium nom. rev.In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List no. 15. Int J Syst Bacteriol 34:355–357 [CrossRef]
    [Google Scholar]
  14. Lambert B., Joos H., Dierickx S., Vantomme R., Swings J., Kersters K., Van Montagu M. 1990; Identification and plant interaction of a Phyllobacterium sp., a predominant rhizobacterium of young sugar beet plants. Appl Environ Microbiol 56:1093–1102
    [Google Scholar]
  15. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  16. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  17. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  18. Mergaert J., Cnockaert M. C., Swings J. 2002; Phyllobacterium myrsinacearum (subjective synonym Phyllobacterium rubiacearum ) emend. Int J Syst Evol Microbiol 52:1821–1823 [CrossRef]
    [Google Scholar]
  19. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
    [Google Scholar]
  20. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int J Syst Bacteriol 30:225–420 [CrossRef]
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. Methods Gen Mol Bacteriol 25:607–654
    [Google Scholar]
  22. Soto-Hernandez J. L., Nunley D., Holtsclaw-Berk S., Berk S. L. 1988; Selective medium with DNase test agar and a modified toluidine blue O technique for primary isolation of Branhamella catarrhalis in sputum. J Clin Microbiol 26:405–408
    [Google Scholar]
  23. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  24. Strimmer K., von Haeseler A. 1996; Quartet puzzling: a quartet maximum-likelihood method for reconstructing tree topologies. Mol Biol Evol 13:964–969 [CrossRef]
    [Google Scholar]
  25. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
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